摘要
４２例肾母细胞瘤石蜡包埋组织经流式细胞仪（ＦＣＭ）ＤＮＡ含量测定显示，二倍体１３例，四倍体１２例，异倍体１７例，异倍体检出率为４０．４８％。二倍体的２年及５年生存率最高，分别为８４．００％和７５．１６％；四倍体次之，均为５５．９０％；异倍体最低，分别为４７．０６％和４１．１８％。二倍体的２年及５年生存率明显高于异倍体（Ｐ＜０．０３和Ｐ（０．０５），尤其在Ⅰ＋Ⅱ期中，二倍体＋四倍体、与异倍体的２年及５年生存率的差异有非常显著性意义（Ｐ＜０．００４和Ｐ＜０．００２）。异倍体对Ⅰ、Ⅱ期患儿的不良预后具有独特的提示作用。
DNA content in extracted nuclei was measured by procedure of Flow Cytometry (FCM) with paraffin-embedded tissue from 42 cases of Wilms' tumor. DNA histograms showed diploid in 13 , tetraploid in 12 and aneuploid in 17 patients. The detectable rate of DNA aneuploid was 40. 48%. The 2 and 5-year survival rate were 84% and 75. 16% respectively in patients with DNA diploid pattern, while petients with DNA aneuploid showed worse prognosis with the 2 and 5-year survival rare of 47. 06% and 41. 18% respectively (P<0. 03 and P<0. 05). The 2 and 5-year survival rate between DNA diploid tetraploid and DNA aneuploid was even more evident(P<0. 004 and P<0. 002). In conclusion,the DNA aneuploid may be an important indicator of poor prognosis in Wilms' tumor,especially,of the stage Ⅰand Ⅱ.
出处
《中华小儿外科杂志》
CSCD
1994年第1期13-15,共3页
Chinese Journal of Pediatric Surgery
