摘要
目的:观察尼氟灭酸(niflumicacid,NFA)对大鼠心室肌细胞钠电流及动作电位的影响。方法:分别用全细胞膜片钳及电流钳技术记录单个心室肌细胞电压门控钠电流(INa)和动作电位(AP)。结果:尼氟灭酸(100μmol/L)能可逆性地抑制INa和AP,与对照组比较,抑制的程度分别为:60.1%±12.1%(-30mV,n=6,P<0.01)及78.2%±10.3%(n=5,P<0.01)。天冬氨酸根离子(Asp-)替代细胞外液的氯离子可引起类似尼氟灭酸的作用。结论:尼氟灭酸对钠通道有抑制作用并影响动作电位。
AIM: To study the effects of niflumic acid on the current of Na^+ channel and action potential in islated rat cardiac ventricular myocytes. METHODS: Whole-cell patch-clamp and current-clamp techniques were used to measure the voltage-gated Na~ current (I_(Na)) and action potential (AP) respectively in single rat cardiac ventricular myocytes. RESULTS: Niflumic acid (NFA, an anion channel blocker, reversibly inhibited I_(Na) and AP. In comparison with the control, NFA decreased I_(Na )and AP amplitude (APA) by 60.1%±12.1% (-30 mV,n=6, P<0.01) and 78.2%±10.3%(n=5, P<0.01) respectively. The inhibitory effects of NFA on I_(Na )and AP were mimicked by replacing the extracellular Cl^- with less permeable anion, aspartate (Asp^- ). CONCLUSION: These data suggested that niflumic acid can inhibit Na~ channel, and influence action potential.
出处
《心脏杂志》
CAS
2005年第3期201-203,206,共4页
Chinese Heart Journal
基金
国家自然科学基金资助项目(30270602)
