期刊文献+

罗格列酮对高糖大鼠肾小管上皮细胞表达转化生长因子β1和纤维连接蛋白的影响

Effects of rosiglitazone on the expression of transforming growth factor β1 and fibronectin in rat renal tubular epithelial cells cultured in high glucose
下载PDF
导出
摘要 目的:观察罗格列酮(rosiglitazone,RGZ)对高糖环境下大鼠肾小管上皮细胞(NRK52E)转化生长因子β1(TGF_β1)和纤维连接蛋白(FN)表达的影响,初步探讨罗格列酮对糖尿病肾病的保护作用。方法:体外培养大鼠肾小管上皮细胞,分成正常对照组NG(含1000mg/LD_葡萄糖)、高糖组HG(含4500mg/LD_葡萄糖)、HG+RGZ(5μmol/L)组、HG+RGZ(10μmol/L)组和HG+RGZ(15μmol/L)组,用蛋白印迹的方法(Westernblotting)检测TGF_β1和FN蛋白表达的改变。结果:HG组细胞TGF_β1和FN的蛋白水平较NG组显著升高,加入罗格列酮后,TGF_β1和FN的表达较HG组降低。结论:罗格列酮可以抑制高糖诱导的小管细胞TGF_β1和FN高表达,具有一定的肾保护作用。 Objective: To investigate the protective effects of rosiglitazone on rat renal tubular epithelial cells(NRK52E) damaged by high glucose.Methods: NRK52E cells were cultured in vitro,and were divided into five groups: normal glucose group(NG,1000 mg/L D-glucose),high glucose group(HG,4500 mg/L D-glucose),HG+RGZ(5μmol/L),HG+RGZ(10 μmol/L) and HG+RGZ(15 μmol/L).The expression of TGF-β1 and FN was examined by Western blotting.Results: Compared with NG group,expression of TGF-β1 and FN increased significantly in HG group,rosiglitazone inhibited those changes.Conclusion: Rosiglitazone can reduce the overexpression of TGF-β1 and FN induced by high glouse,has protective effects on kidney.
出处 《河南医学研究》 CAS 2005年第2期104-106,共3页 Henan Medical Research
基金 河南省科技攻关项目(324410084)。
关键词 罗格列酮 高糖 肾小管上皮细胞 转化生长因子Β1 纤维连接蛋白 糖尿病肾病 rosiglitazone high glucose renal tubular epithelial cells transforming growth factor β1 fibronectin
  • 相关文献

参考文献9

  • 1Tsuchiya T, Shimizu H, Shimomura K, et al. Troglitazone Inhibits Isolated Cell Proliferation, and Induces Apoptosis in Isolated Rat Mesangial Cells[J].Am J Nephrol,2003,23(4) :222-228.
  • 2刘章锁,牛红心,程根阳,李军辉,宋洁.罗格列酮对糖尿病大鼠肾保护机制的研究[J].中华肾脏病杂志,2004,20(2):109-112. 被引量:35
  • 3刘章锁,程根阳,宋洁,李军辉.罗格列酮对单侧输尿管梗阻大鼠肾脏的保护作用[J].中华肾脏病杂志,2004,20(4):268-272. 被引量:15
  • 4Phillips AO, Steadman R, Morrisey K, et al. Exposure of human renal proximal tubular cells to glucose leads to accumulation of type Ⅳ collagen and fibronectin by decreased degradation [ J ]. Kidney Int, 1997,52 (4): 973-984.
  • 5Eddy AA. Molecular basis of renal fibrosis [ J ]. Pedistr Nephrol, 2001,15(3) :290-301.
  • 6Cheng J, Grand JP. Transforming gxowth factor beta signal transduction and progressive renal disease [ J ]. Exp Biol Med, 2002,227 ( 11 ): 943-956.
  • 7Bottinger EP,Bitzer M.TGF beta signaling in renal disease[J] .J Am Soc Nephrol,2002,13(10): 2600-2610.
  • 8Zhang Z, Kundu GC, Yuan CT, et al. Severe fibronectin deposit renal glomerular disease in mice lacking uteroglobin [ J ]. Science, 1997, 276(5317): 1408-1412.
  • 9Asano T,Wakisaka M,Yoshinnari M,et al. Peroxisome proliferator activated receptor gamma 1 ( PPARγ1 ) expresses in rat messengial cells and PPARγlagonists modulate its differentiation [ J]. Biochim Biophys Acta,2000,1497( 1 ): 148-154.

二级参考文献25

  • 1Li-jun Ma, Marcantpni C, Linton MF, et al. Peroxisome proliferatoractivated receptor-γagonist troglitazone protects against nondiabetic glomerulosclerosis in rats. Kidney Int, 2001, 59: 1899- 1910.
  • 2Parameswaran N, Hall CS, Bomberger JM. Negative growth effects of ciglitazone on kidney interstitial fibroblasts: role of PPAR-gamma. Kidney Blood Press Res, 2003, 26: 2-9.
  • 3Wright EJ, McCaffrey TA, Robertson AP, et al. Chronic unilateral ureteral obstruction is associated with interstitial fibrosis and tubular expression of transforming growth factor-beta. Lab Invest,1996, 74: 528-537.
  • 4Galli A, Crabb DW, Ceni E, et al. Antidiabetic thiazolidinediones inhibit collagen synthesis and hepatic stellate cell activation in vivo and in vitro. Gastroenterology, 2002, 122: 1924-1940.
  • 5Ricote M, Li AC, Willson TM, et al. The peroxisone proliferator activated receptor y1 is an negative regulator of macrophage activation. Nature, 1998, 391: 79-82.
  • 6Jiang C, Ting AT, Seed B. PPARγagonists inhibit production of monocyte inflammatory cytokines. Nature, 1998, 391: 82.
  • 7Max N, Mach F, Sauty A, et al. Peroxisome proliferator activated receptor gamma activators inhibit IFN gamma induced expression of the T cell active CXC chemokines IP 10, Mig, and I TAC in the human endothelial cells. J Immunol, 2000, 164: 6503-6508.
  • 8Yang XY, Wang LH, Chen T, et al. Activation of human T1lymphocytes is inhibited by peroxisome proliferator activated receptor γ(PPARγ)agonists. J Biol Chem, 2000, 275: 4541-4544.
  • 9Su GG, Wen X, Bailey ST, et al. A novel therapy for colitis utilizing PPARγ ligands to inhibit the epithelial inflammatory response. J Clin Invest, 1999, 104: 383-389.
  • 10Galli A, Crabb DW, Ceni E, et al. Antidiabetic thiozolidinediones inhibit collagen synthesis and hepatic stellate cell activation in vivo and in vitro. Gastroenterology, 2002, 122: 1924-1940.

共引文献43

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部