摘要
目的:检测肾病综合征(NS)患者肾小球组织中nephrin的表达,以探讨nephrin在肾病综合征患者蛋白尿发生机制中的可能作用。方法:免疫荧光染色图像分析及RT- PCR法检测NS、单纯血尿患者及正常对照组肾小球组织nephrin的表达水平。结果:免疫荧光染色定量分析NS患者肾小球组织nephrin的表达量显著低于单纯血尿患者(P<0 .0 5 )及正常对照组(P<0 .0 5 )。并且NS患者肾小球组织中nephrin的m RNA表达量也显著低于单纯血尿患者(P<0 .0 5 )及正常对照组(P<0 .0 5 )。结论:NS患者肾小球组织nephrin的表达减少,可能影响肾小球滤过屏障的通透性,导致蛋白尿的产生。
Objective:To examine the expression of nephrin in the glomerular and to investigate the possible role of nephrin in the development of proteinuria in patients with nephrotic syndrome (NS).Method:Immunofluorescence staining,confocal laser scanning microscopy and RT-PCR analysis were used to investigate the expression of the nephrin in the glomerular in patients with NS,isolated hematuria and control group.Result:In the patients with NS,immunofluorescence staining analysis found that the glomerular expression of nephrin decreased significantly compared with that in isolated hematuria patients(P<0.05) or control group(P<0.05).And what′s more, the glomerular expression of the nephrin mRNA also decreased significantly compared with that in isolated hematuria patients(P<0.05)or control group(P<0.05).Conclusion:The results showed that the dramatic decrease of nephrin expression might contribute to the development of proteinuria in the patients with NS.
出处
《中国误诊学杂志》
CAS
2005年第5期816-818,共3页
Chinese Journal of Misdiagnostics
关键词
肾病综合征
代谢
肾小球
蛋白尿
Nephrotic syndrome/metabolism
Kidney glomerulus/metabolism
Proteinuria
