摘要
首次应用生物素－１４－ｄＵＴＰ标记Ｒｂ３．８ｋｂ探针，结合亲合素－碱性磷酸酶发光及自显影法，对Ｒｂ基因在鼻咽癌中存在状态进行了研究。杂交结果表明，３例正常胎儿鼻咽组织出现分子量为１２．８、１０．２、８．０、６．２、５．６、５．２及４．８ｋｂ的７条杂文区带，１１例鼻咽癌组织均发现有Ｒｂ基因缺失或失活，其中４例为５．６ｋｂ片段缺失，４例为４．８ｋｂ缺失并有２例伴５．６ｋｂ减弱，２例为１０．２ｋｂ缺失，１例为５．６及５．２ｋｂ片段显著减弱，说明在上述１１例鼻咽癌中均有Ｒｂ基因的改变。这种高频率的异常变化，提示Ｒｂ基因的缺失或失活，与鼻咽癌的发生有密切关系。
The status of Rb gene in nasopharyngeal carcinoma(NPC)and normal fetal nasopharynx tissues were studied by using biotin-14-dUTP labeled Rb cDNA 3.8kb probe in combination with streptavidin alkaline phosphatase luminescent detection system.Seven hybridizing bands of 12.8,10.2,8.0,6.2,5.6,5.2 and 4.8kb in DNA from 3 cases of normal fetal nasopharynx tissues as controls were observed.The structural abnormal alterations of Rb gene in DNA from 11 cases of NPC were studied,in which ob served were the deletion of 5. 6kb fragment in 4 cases,deletion of 4.8kb fragnient in 4 cases,deletion of 10.2kb fragment in 2 cases and the obvious loss of activity of both 5.6 and 5.2kb fragment in 1 case.The results implied that the alterations of the deletion or loss of activity of Rb gene with high frequency might be associated with the carcinogenesis of NPC.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1994年第2期75-77,共3页
Chinese Journal of Medical Genetics
关键词
RB基因
鼻咽肿瘤
癌
基因缺失
检测
Nasopharyngeal carcinoma Rb gene Gene deletion Luminescent detection system
