摘要
为了提高淋巴因子激活的杀伤细胞（ＬＡＫ细胞）对乙肝病毒感染细胞的杀伤活性，研究了白细胞介素２（ＩＬ２），白细胞介素４（ＩＬ４）和肿瘤坏死因子（ＴＮＦ）单独及不同组合时，所诱生的ＬＡＫ细胞对ＨｅｐＧ２细胞和２２ｌ５细胞的杀伤活性，并初步研究了ＬＡＫ细胞的活化及杀伤机制。结果表明：三种细胞因子的组合诱导较两种细胞因子组合好，而两两组合又较单独诱导好；另外，ＩＬ２单独诱导时，随其剂量的增加，细胞表面ＩＬ２受体（ＩＬ—２Ｒ）表达量也增加，细胞内溶酶体含量也增加，其对靶细胞的杀伤活性也增加，均呈正相关。提示ＩＬ—２Ｒ和溶酶体可能参与ＬＡＫ细胞的活化和杀伤机制。
e studied the effect of single or combination ofinterleukin-2 (IL-2), interleukin-4 (IL-4) and tumornecrosis factor (TNF) on lymphokine-activated killercells (LAK) activity which killed both of target cellsHepG2 and 2 2,l,5 cells induced for seven days. It wasshown that LAK activity induced by the combinationof triplet of IL-2, IL-4 and TNF was higher than thatinduced by single or two of IL-2, IL-4 and TNF, andthat enhanced LAK activity was associated with thequantity of IL-2 receptor expression and lysosomes ofLAK precursors.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1994年第4期235-237,共3页
National Medical Journal of China
关键词
白细胞介素
肿瘤坏死因子
杀伤细胞
Interieukin-2 Interleukin-4Tumor necrosis factor LAK activity
