摘要
目的探讨子宫颈腺癌组织中环氧化酶2(COX2)与细胞周期调控因子p27kip1、基质金属蛋白酶2(MMP2)的表达关系。方法采用组织微阵列技术结合免疫组化SP法检测106例子宫颈腺癌组织和22例慢性子宫颈炎组织中COX2与p27kip1、MMP2的表达。结果106例子宫颈腺癌组织COX2和MMP2的阳性表达率分别为86.8%和70.8%,均高于慢性子宫颈炎组织(P<0.01);p27kip1阳性表达率为58.5%,低于慢性子宫颈炎组织81.8%(P<0.05)。COX2和MMP2在子宫颈腺癌的病理分级中,G3组阳性表达率均明显高于G1组(P<0.05)。MMP2和p27kip1表达与子宫颈腺癌组织学类型有关,透明细胞腺癌MMP2阳性表达率高于子宫颈内膜腺癌和子宫内膜样腺癌(P<0.05),p27kip1阳性表达率低于子宫颈内膜腺癌和子宫内膜样腺癌(P<0.05)。COX2阳性表达强度与MMP2的阳性表达强度呈正相关(P<0.01)。COX2阳性表达强度与p27kip1的阳性表达强度呈负相关(P<0.05)。结论COX2与p27kip1、MMP2在子宫颈腺癌组织中的表达密切相关,p27kip1低表达与COX2、MMP2增强表达在子宫颈腺癌的发生发展中起重要作用,三者可能作为子宫颈腺癌恶性化的分子标志。
Purpose To study the relationship of the expression of cyclooxygenase-2 (COX-2) with cell cycle regulator p27^(kip1) and matrix metalloproteinases-2 (MMP-2) in cervical adenocarcinoma. Methods The expression of COX-2 with p27^(kip1) and MMP-2 was determined by tissue microarray combined with immunohistochemistry in 106 cases of cervical adenocarcinoma and 22 cases of cervical chronic inflammation. Results The positive rates of COX-2 and MMP-2 in cervical adenocarcinoma were 86.8% and 70.8% respectively,which were higher than that in cervical chronic inflammation(P<0.01). The positive rates of p27^(kip1) in cervical adenocarcinoma was 58.5%,which was lower than that in cervical chronic inflammation(P<0.05). The positive expression of COX-2 and MMP-2 was significantly higher in G3 than in G1 adenocarcinoma(P<0.05). The expression of MMP-2 and p27^(kip1) was related to the histologic types,the level of MMP-2 was higher in clear-cell adenocarcinoma than that in endocervical adenocarcinoma and endometrioid adenocarcinoma (P<0.05). While p27^(kip1) was lower in clear-cell adenocarcinoma than others (P<0.05). There was positive correlation between the expression of COX-2 and MMP-2 in cervical adenocarcinoma(P<0.01),but negative correlation between the expression of COX-2 and p27^(kip1)(P<0.05). Conclusions Low-expression of p27^(kip1) and over-expression of COX-2 and MMP-2 are closely related in cervical adenocarcinoma and they play an important role in the development of cervical adenocarcinoma. The three biomarkers might be the indicators for the transformation of cervical lesions from benign to malignant status.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2005年第3期303-307,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
湖南省教育厅青年科研基金(04B048)
