摘要
以人皮和豚鼠皮新鲜及-196℃冷冻皮片匀浆做为抗原,研制出免抗人和豚鼠的新鲜及-196℃冷冻皮片匀浆的可溶性蛋白高效价的免疫血清,并通过免疫电泳、火箭免疫电泳、免疫火箭扩散电泳及单相免疫扩散电泳等不同免疫学电泳的检测,分别对新鲜皮片及-196℃冷冻皮片抗原性改变的这一现象进行探讨与研究。结果表明:人皮、豚鼠皮其新鲜皮片的抗原成份、抗原决定簇及抗原量均显著高于相应的-196℃冷冻组皮片,因而证实了通过深低温冷冻的皮片其抗原性低于新鲜皮片的论断。
It is well accepted impression that antigenic properties of skin are low- ered after cryopreservation.In order to prove it,this experiment was done.Fresh guinea pig and cadaveric skin homogenates with appropriate adjuvants were injected hypodermi- cally separately to rabbits once a week.Five weeks later,blood was withdrawn from rab- bits,thus anti-guinea pig skin serum(AGS)and anti-cadaveric skin serum(ACS)were obtained.Homogenates of fresh guinea pig skin(FG)and guinea pig skin stored at -196℃ (SG),homogenates of fresh cadaveric skin(FC)and cadaveric skin stored at -196℃(SC) were used as antigens.Microimmunoelectrophoresis and rocket immunoelectrophoresis were performed between related antigens and antibodies.The results of microimmu- noelectrophoresis showed that there were four dense and wide precipitated are lines in AGS vs FG and ACS vs FC groups,while only two light and thin precipitated are lines in AGS vs SG and ACS vs SC groups.Rocket immunoelectrophoresis showed that the rock- et precipitated area in AGS vs FG and ACS vs FC groups are larger (longer in axis,and broader in width)than in AGS vs SG and ACS vs SC groups.The antigenicity of skin is lowed after being stored at -196℃ as compared with that of the fresh one as shown by electrophoresis method.
关键词
免疫学
血清
抗原性
冷冻
皮肤
Immunity
Serum
Antigenicity
Skin cryopreservation
