摘要
Transgenic poplar(Populus alba×P.glandulosa cv`84k’) plants with the coleopterous insect resistant genes (BtCry3A and OC-I)were obtained. The transgenic nature of these plants was confirmed by PCR amplification and dot hybridization. The transgenic poplar’s toxicity towards the Anoplophora glabripennis larvae was assessed on two year-old selected plants in laboratory conditions. The results indicated that the transgenic lines BOGA-38 and BOGA-39 were deleterious for A. glabripennis larvae, and BOGA-5、BOGA-31、BOGA-38、BOGA-39 could inhabit the growth of A. glabripennis larvae. BOGA-39 was the most toxic one among the transgenic lines, with (41.18 %) corected mortality rate and 78.90 % growth inhabit rate for the larvae.
Transgenic poplar(Populus alba×P.glandulosa cv`84k') plants with the coleopterous insect resistant genes (BtCry3A and OC-I)were obtained. The transgenic nature of these plants was confirmed by PCR amplification and dot hybridization. The transgenic poplar's toxicity towards the Anoplophora glabripennis larvae was assessed on two year-old selected plants in laboratory conditions. The results indicated that the transgenic lines BOGA-38 and BOGA-39 were deleterious for A. glabripennis larvae, and BOGA-5、BOGA-31、BOGA-38、BOGA-39 could inhabit the growth of A. glabripennis larvae. BOGA-39 was the most toxic one among the transgenic lines, with (41.18 %) corected mortality rate and 78.90 % growth inhabit rate for the larvae.
出处
《林业科学研究》
CSCD
北大核心
2005年第3期364-368,共5页
Forest Research
基金
国家转基因植物研究与产业化专项课题--优质高产抗干旱耐盐碱杨树基因工程育种研究(J2002 B 004)
关键词
杨树
遗传转化
双价基因
蛀干害虫
抗虫性
Populus
transformation
binary genes
coleopterous insect
insect resistance
