摘要
应用无血清液体一半固体二次培养法，观察了重组人干细胞因子（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｓｔｅｍｃｅｌｌｆａｃｔｏｒ，ｒｈＳＣＦ）对人类急性髓系白血病干／祖细胞（ＡＭＬ－ＣＦＵ）自我更新的影响。认为ｒｈＳＣＦ是一作用阶段早于白介素３（ＩＬ－３）和粒／巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）的造血生长因子（ＨＧＦ）。无论单一，还是与其它ＨＧＦ联合，ｒｈＳＣＦ对绝大部分ＡＭＬ患者，均有强大的维持其ＡＭＬ－ＣＦＵ自我更新的作用。但不同ＦＡＢ亚型及ＦＡＢ亚型相同的患者之间，这种维持作用差异较为显著。提示ＳＣＦ在ＡＭＬ病理过程中起重要作用。
Abstract Using serum- free culture system and liquid/sem-solid dual culture technque, we examinedthe effect of rhsCF on self-renewal of the stem/progenitor cells of human acute myelogenousleukemia(AML- CFU).The results revealed that the rhSCF as a hematopoietic growth factor(HGF)exerted its effect hierarchically earlier than interleukin- 3and granulocyte/macriophage colo-ny-stimulating factor on AM L- CFU. rhSCF, whether used alone or in combination with otherHGFs, showed potential maintenance of self- renewal of AML- CFU in most AML patientsstudied. However,considerable discrepancy existed in maintenance among various FAB types of pa-tients or even in patients with the same FAB type. It is suggested that SCF may play an impor-tant role in the pathological development of AML.
出处
《中华肿瘤杂志》
CSCD
北大核心
1994年第4期259-262,共4页
Chinese Journal of Oncology
