摘要
目的应用随机扩增多态DNA(RAPD)技术对近交系中国地鼠山医群体的遗传结构进行多态分析.方法采用30条10 bp引物对中国地鼠山医群体的A家系和E家系共24个个体进行RAPD扩增,分析家系的遗传纯度,探讨两个家系的亲缘关系.结果30条随机引物对供试的24个个体产生255条谱带.单个引物扩增的谱带数是2～17条,平均8条.所有样品的相似系数0.943 1～0.995 5之间变动,平均相似系数为0.974 9.根据扩增出的RAPD图谱,运用SPSS软件包中的Wardmethod法进行聚类分析.结论供试中国地鼠A家系和E家系两个家系间存在一定程度的差异,中国地鼠A家系和E家系分别先聚为一类,中国地鼠群体间的分子聚类关系与各群体间的亲缘关系基本一致.
Objective To analyze genetic constuction of Chinese hamster from Shanyi colony inbred with random amplified polymorphic DNA(RAPD) markers. Methods Twenty-four hamsters of Shanyi colony were analyzed with 30 primers,then studied genetic relationships between A genealogy and E genealogy. Results ①A total of 255 clear and reproducible bands were generated, and obtaining segments of individual primer were among 2～17, on average, about 8 bands per primer. ②The amplified fragment bands were analyzed to calculate the similarity index. The similarity index of all samples ranged from 0.943 1 to 0.995 5, averagely at 0.974 9. Chinese hamster of Shanyi colony A and E genealogy has reached a high degree of genetic purity. ③The dendrogram based on 255 RAPD markers was constructed and clustered using Ward method. The cluster analysis indicated strong similarities within genealogy, firstly, the individuals in each genealogy closely clustered together. Conclusion The molecular dengrogram among Chinese hamster was in accord with their genetic relationship.
出处
《山西医科大学学报》
CAS
2005年第3期270-274,共5页
Journal of Shanxi Medical University
基金
科技部基础性工作专项资金资助项目(2002DAE10010)
