摘要
目的研制2型糖尿病大鼠模型。方法新生2d龄Wistar大鼠147只,随机分为造模组和对照组,造模组大鼠90mg/kg腹腔注射链脲佐菌素,断乳后再次25mg/kg追加一次,并应用高糖高脂膳食诱导建立2型糖尿病大鼠模型。结果从生化指标和形态学方面证实该方法可以成功制备2型糖尿病大鼠模型。结论该方法制备的2型糖尿病大鼠模型,造模时间短,成模率高,模型稳定。
Objective To explore a method to develop the type 2 diabetes mellitus (T2DM) rat model. Methods Two-day-old Wistar rats (n=147) were divided into two groups: model and control groups. The modol group rats underwent intraperitoneal injection with 90 mg/kg streptozotocin (STZ). When they had ablacted they were injected with 25 mg/kg streptozotocin(STZ) again. Then these rats were fed the diets enriched with sucrose (20%) , lard(18%) and yolk powder(3%).Another group served as control group. Results Biochemical parameters and morphology proved that the methods could develop the T2DM rat model. Conclusion This method to develop T2DM rat model is high-efficient, stable and quick.
出处
《山西医科大学学报》
CAS
2005年第3期275-279,共5页
Journal of Shanxi Medical University
基金
山西省自然科学基金资助项目(20021112)
山西省归国留学人员基金资助项目(2002[16]39)
