摘要
[目的]了解16SrRNA基因在细菌区分中的应用价值。[方法]细菌热裂解后用荧光(FAM,JOE)标记引物进行扩增,产物用HaeIII消化并进行毛细管电泳。[结果]所有细菌PCR产物均产生255bp片段,酶切产物电泳后具有科间差别。[结论]该方法可以用于不同细菌之间的分科。
[Objective] In order to appraise the utility of 16S rRNA gene for the differentiation of different bacte- ria, a pair of primers were selected to amplify this gene. The PCR products were then digested by restriction en- donuclease. [Method] All the bacteria were lysed by heat and amplified by two kinds of fluorescence -FAM and JOE, respectively, labeled primers. The PCR product were digested by Hae III and then taken capillary electrophoresis. [Result] All the bacteria can generated a 255bp product after amplification. The products di- gested were different in different families. [Conclusion] This method can be applied to the practice of differenti- ation of different bacteria belonging to different families.
出处
《大连医科大学学报》
CAS
2005年第3期237-239,共3页
Journal of Dalian Medical University
