摘要
采用热水法提取罗汉果多糖,用Sevag法去除蛋白质,通过用DEAE 纤维素柱和SephadexG 200柱的分离纯化得到2个多糖组分。经测定,其相对分子质量分别为Mr1=430000,Mr2=650000。组分1的单糖组成:葡萄糖、半乳糖、木糖、阿拉伯糖、鼠李糖和葡萄糖醛酸;组分2的单糖组成;鼠李糖、葡萄糖醛酸。
The extraction of polysaccharides from siraitia grosvenorii (SGPS) was studied. The crude polysaccharides was purified by chromatographic columns of DEAE-cellulose and Sephadex G-200 to get SGPS1 and SGPS2, respectively. According to TLC, SGPS1 was composed of D-glucose, D-galactose, D-xylose, L-arabiose, L-rhamnose and glycuronic acid. SGPS2 was composed of L-rhamnose and glycuronic acid. Their homogenity and molecular mass were measured by HPLC. Relative molecular mass of SGPS1 and SGPS2 were 430000 and 650000, respectively.
出处
《化学世界》
CAS
CSCD
北大核心
2005年第5期277-280,共4页
Chemical World
基金
广西省高校基金资助项目(桂教科研2002第316号)
关键词
罗汉果
多糖
分离纯化
Siraitia grosvenorii
polysaccharides
extraction
