摘要
目的 探讨海藻多糖对人HL 60细胞增殖的影响。方法 采用集落培养、MTT、形态观察等方法,研究海藻多糖对(HL 60)体外增殖的影响。结果 海藻多糖对体外培养的HL 60细胞的抑制作用随时间和浓度的增加而增强,MTT结果示当浓度为800μg/ml,作用48h时抑制率为(51 30±3 10)%。结论 海藻多糖体外可抑制HL 60细胞增殖,提示其在抗肿瘤方面有一定的开发应用前景。
Objective To explore the effect of seaweed polyaccharide on the proliferation in the HL-60 cell line.Method The MTT,clone forming approaches and cell morphology were adopted.Results Seaweed polyaccharide can inhibit the proliferation of HL-60 cells in a dose- and time-dependent manner in a certain range of dose (0～800μg/ml).After being treated with the polysaccharide (800 μg/ml) for 48 h,the inhibited rate is (51.30±3.10)%.Conslusion The seaweed polysaccharide can inhibit the HL-60 cell proliferation,suggesting it can be used as an antileukemia drug.
出处
《滨州医学院学报》
2005年第2期87-88,共2页
Journal of Binzhou Medical University
