摘要
目的对3种胶体金免疫层析测定(GICA)试条检测HBsAg的性能、结果等技术参数进行初步探讨,为各实验室的选用提供参考.方法采用GICA法和酶联免疫吸附法(ELISA)对定值的HBsAg质控品和1 322份血清标本同时测定,并结合有关实验,对3种GICA试条的敏感性、特异性、渗透性、固相膜均匀性等结果进行初步评价.结果 3种GICA试条对HBsAg的最小检出量为1～5ng/ml;与ELISA法比较,检出率为97.37%～99.57%,一般不出现假阳性,未发现前带反应.结论 GICA适用于急诊检验,其敏感性低于ELISA法,成本高;特殊情况下,还必须与ELISA法并用,实行双检.
Objective To prove the quality and performance of 3 sources of gold immunochromatography assay(GICA) strips for HBsAg detection and to provide data for various laboratiories in the selection of HBsAg assay methods.Methods A few known molar manipulative serum and 1 322 serum samples were tested with both GICA strips and ELISA.The sensitivity,specificity of the strips and pertinent experiment were assessed.Results The minimal detection limits of the 3 sources of GICA strips were 1-5ng/ml,with detection rates varying from 97.37 to 99.57%,as compared with ELISA.No false positives and pre-band phenomena were found.Conclusion GICA is fit emergency.lt is less sensitive than ELISA,and costs more.On the unusual condition,it is should be combined with ELISA.
出处
《国外医学（临床生物化学与检验学分册）》
2005年第3期141-142,共2页
Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine
