摘要
用RSM方法对黄杆菌属产红色素菌株S9801(Flavobacteriumsp.)的培养基进行了优化.首先用部分重复因子实验对培养基组分蛋白胨、酵母粉、葡萄糖及NaCl浓度对菌株产红色素的影响进行评价,找出主要影响因子为葡萄糖和NaCl浓度.两者均为正影响,其他组分对色素含量的影响不显著.第二步用最陡爬坡路径逼近最大响应区域.最后用中心组合设计及响应面分析确定主要影响因子的最佳浓度.菌株在优化培养基中培养较初始培养基色素含量提高320%.
Response surface methodology was used to optimize a medium for pigment production of marine bacterium S-9801 strain. In the first optimization step the influence of yeast extract,peptone, glucose and sodium chloride on pigment production was evaluated using a fractional factorial design.Glucose and sodium chloride influenced pigment production positively while other components had no significant effect. The path of steepest ascent was used to approach the optimal region of the medium composition. In the third step the optional concentration of glucose and sodium chloride was determined by a central composite design and response surface analysis.The optimized medium allowed pigment production(OD_(535~650))to be increased from 463 to 559, 320% higher than the original medium.
出处
《海洋学报》
CAS
CSCD
北大核心
2005年第3期142-146,共5页
基金
国家"863"资助项目(2001AA620402)
国家海洋局科技兴海资助项目(2000112)
青岛市科技重点资助项目(JXM0111).
关键词
RSM方法
培养基优化
色素
response surface methodology
red pigment
optimization
