摘要
目的:探讨氢化可的松在白细胞介素1α(IL - 1α)诱导的角膜基质细胞降解中的作用。方法:利用角膜基质细胞三维胶原凝胶表面添加纤维蛋白溶酶原(0 .0 6 μg·L- 1 )、不同浓度的IL- 1α(0、0 .1、1.0、10 .0 μg·L- 1 )及IL- 1α(10 μg·L- 1 )与不同浓度的氢化可的松(0、1、10、10 0 m mol·L- 1 ) ,在MEM培养液中培养2 4 h后,通过测定培养上清液中的羟脯氨酸(HYP)的量作为胶原降解的活性单位。结果:在存在纤维蛋白溶酶原的情况下,随着IL- 1α浓度的升高,HYP含量增加,IL- 1α0 .1、1.0和10 .0 μg·L- 1组与对照组比较及各剂量组间比较,差异均有显著性(P<0 .0 2 5或P<0 .0 5 ) ;在存在纤维蛋白溶酶原、IL- 1α(10 μg·L- 1 )的情况下,随着氢化可的松质量浓度的增加,HYP含量减少,氢化可的松1、10、10 0 mm ol·L- 1 组与对照组(0 mm ol·L- 1 )比较及各剂量组间比较,差异均有显著性(P<0 .0 2 5或P<0 .0 5 )。结论:氢化可的松具有抑制IL - 1α诱导的角膜基质细胞胶原降解的作用。
Objective To investigate the effects of hydrocortisone on degradation of keratocyte induced by interleukin-1α(IL-1α). Methods The keratocyte-collagen gels were incubated with plasminogen (0.06 μg·L -1 ) and different concentrations of IL-1α(0,0.1,1.0, and 10.0 μg·L -1 ) or interleukin-1α (10 μg·L -1 ) and different concentrations of hydrocortisone(0,1,10, and 100 mmol·L -1 ) or in MEM culture medium separately 24 h later, to evaluate the collagen degradation level, and the amount of hydroxyproline(HYP) were measured. Results In the presence of plasminogen, the amount of HYP was increased with the increasing of IL-1α concentration.There were significant differences(P<0.025 or P<0.05)between 0.1,1.0,and 10.0 μg·L -1 IL-1α groups and control group(0 μg·L -1 ),between 1.0,10.0 μg·L -1 groups and 0.1 μg·L -1 group,between 10.0 μg·L -1 group and 1.0 μg·L -1 group. In the presence of plasminogen and IL-1α(10 μg·L -1 ), the amount of HYP was increased with the increasing of hydrocortisone concentration. There were significant differences(P<0.025 or P<0.05)between 1,10,100 mmol·L -1 hydroxyproline groups and control group (0 mmol·L -1 ), between 10, 100 mmol·L -1 groups and 1 mmol·L -1 group,between 100 mmol·L -1 group and 10 mmol·L -1 group. Conclusion Hydrocortisone can inhibit keratocyte-collagen degradation induced by IL-1α.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2005年第3期395-397,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划项目资助课题 (2 0 0 10 5 2 4 )
