摘要
为设计合成具有应用前景的低毒抗肿瘤药物,本文合成了1种未见报道的铜(Ⅱ) 苏氨酸 咪唑混配配合物。根据元素分析、红外光谱、差热 热重分析及摩尔电导率的测定对其进行表征;并以电子吸收光谱法及溴化乙锭(EB)荧光分析法研究了此种配合物与DNA的作用方式。结果表明:配合物[Cu(Thr) (Im ) 3 ]Cl·H2 O(HThr =DL -Threonine ,Im =Imi dazole)与DNA作用时,使DNA电子吸收光谱的吸收峰发生微小位移,但该配合物在一定程度上又能够猝灭EB DNA体系的荧光,表明此配合物与DNA是以弱的插入作用相结合。
A new complex of [Cu(Thr)(Im) 3]Cl·H 2O ( HThr=DL-Threonine, Im=Imidazole,), which has not been published, was synthesized and characterized by elemental analysis, IR spectroscopy, TG-DTA and molar conductance. The interaction of the complex with sperm DNA has been studied by electronic absorption and ethidium bromide (EB) fluorescence spectroscopy. The results show that this complexe can make the absorption peaks of DNA only a little shift. However, it can at some extent quench the emission intensity of EB-DNA system. Therefore, we come to the conclusion that the interaction of [Cu(Thr)(Im) 3]Cl·H 2O in binding with sperm DNA is weak intercalation.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第3期467-470,共4页
Periodical of Ocean University of China
基金
国家自然科学基金项目 (2 0 371 0 4 3)资助
