摘要
观察银杏叶提取物(EGb761)对红细胞脂质过氧化损伤的影响,探讨其保护红细胞的机制。以活性氧(H2 O2 )作用于离体的正常红细胞,观察EGb761对红细胞溶血度、红细胞丙二醛含量(MDA)、红细胞超氧化物歧化酶(SOD)和ATP酶(ATPase)活性及红细胞膜骨架结构的影响。结果表明:EGb761能明显降低红细胞溶血度,且随着浓度增加其作用逐渐增强,与对照组比较差异有极显著性(t =4.13～19.41,P <0 .0 5 ,0 .0 0 1) ;EGb761能明显降低红细胞MDA含量,提高其SOD ,ATPase酶活性,与对照组比较差异有极显著性(t =2 .0 2～70 .0 6,P <0 .0 5 ,0 .0 0 1) ;EGb761能降低过氧化损伤对红细胞膜骨架的破坏,保护其完整性。可见EGb761能在膜水平上直接对抗氧自由基引发的红细胞脂质过氧化损伤,保护红细胞及其膜骨架结构。
The aims of this paper are to observe the effects of Ginkgo biloba extracts(EGb761)on erythrocyte lipid peroxidative injury,and to explore the protective mechanism of EGb761 for erythrocytes. Normal erythrocytes were treated with active oxygen(H 2O 2).The effects of EGb761 on the hemolysis of erythrocytes,the concentration of the product of erythrocyte lipid peroxidation—malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) and ATPase in RBC were measured,and the membrane skeleton structure of erythrocytes was studied under a transmission electron microscope. Compared with the control group,EGb761 could significantly decrease the erythrocyte hemolysis,and the effects were concentration-dependant: greater EGb761 concentrations. had more sig-nificant effects. EGb761 could decrease the erythrocyte MDA concentration and improve the activity of SOD and ATPase,and decrease the injury of erythrocyte membrane skeleton from lipid peroxidation and keep its integrity. EGb761 could,at the membrane level,directly resist the erythrocyte peroxidation caused by oxygen free radicals.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第3期487-490,381,共5页
Periodical of Ocean University of China
