摘要
目的分析产超广谱β内酰胺酶(ESBLs)大肠埃希菌对氨基糖苷类抗生素耐药性及产生的修饰酶种类。方法32株经检测产ESBLs大肠埃希菌用Vitek2系统AST N020药敏试验卡检测,经Vitek2AES(高级专家系统)对庆大霉素、阿米卡星和妥布霉素结果分析,推测可能存在的氨基糖苷修饰酶。结果产ESBLs大肠埃希菌对庆大霉素耐药率为68.75%,妥布霉素为18.75%,但中介为43.75%,对阿米卡星耐药率为6.25%。推测主要氨基糖苷修饰酶为ANT(2″)和AAC(3)Ⅱ,引起庆大霉素和妥布霉素耐药。结论检测氨基糖苷修饰酶可为临床提供更准确的氨基糖苷抗生素治疗信息,以减少此类抗生素耐药性的发生。
Objective To analyze the aminoglycoside-modifying enzymes among ESBL-producing Escherichia coli (E.coli) isolates.Methods Susceptibility of 32 ESBL-producing E.coli to aminoglycoside antibiotics,such as gentamycin,tobramycin and amikacin,was detected by Vitek2 System with AST-N020 gram-negative susceptibility testing card.The type of aminoglycoside-modifying enzymes was referred by Vitek2 AES (Advanced Expert System) according to the pattern of resistance to the aminoglycoside antibiotics.Results The highest resistance rate (68.75%) was found for gentamicin.The resistance rate to tobramycin was 18.75%,but the intermediate resistance was 43.75%.The resistance rate to amikacin was lowest (6.25%).The two main types of aminoglycoside-modifying enzymes were ANT(2″)and AAC(3)-II which were associated with the resistance to gentamycin and tobramycin.Conclusion The detection of aminoglycoside-modifying enzymes may provide more accurate information for effective use of aminoglycosides antibiotics.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2005年第3期180-182,共3页
Chinese Journal of Clinical Laboratory Science
