摘要
目的和方法:利用Fos蛋白和酪氨酸羟化酶(TH)的双重免疫组化方法,观察侧脑室注射肾上腺髓质素对大鼠心血管相关核团中儿茶酚胺神经元及c fos表达的影响,以探讨肾上腺髓质素的中枢效应是否通过激活脑内儿茶酚胺能神经元而诱发。结果:①侧脑室注射肾上腺髓质素(3nmol/kg)诱发脑干、下丘脑及前脑等多个部位的心血管中枢出现大量Fos样免疫反应神经元。②侧脑室注射肾上腺髓质素引起最后区(AP)、孤束核(NTS)、巨细胞旁外侧核(PGL)和蓝斑核(LC)内Fos TH双标神经元明显增加。③降钙素基因相关肽受体拮抗剂CGRP8-37(30nmol/kg)可明显减弱肾上腺髓质素的效应。结论:肾上腺髓质素可兴奋脑干、下丘脑及前脑等多个部位心血管相关核团的神经元,其中枢效应通过激活儿茶酚胺能神经元而诱发,降钙素基因相关肽受体介导这一效应。
Aim and Methods: Using double immunohistochemical method for Fos and tyrosine hydroxylase(TH) to examine the effects of intracerebroventricular(icv) administration of adrenomedullin(AM) on catecholaminergic neurons and the expression of c-fos gene in rat brain nuclei involved in cardiovascular regulation in order to define whether the effects of central administration of adrenomedullin(AM) were induced by activating the catecholaminergic neurons. Results: ① Following icv administration of AM(3 nmol/kg), Fos-like immunoreactivity neurons were markedly increased in several brain areas of the rat, including the brainstem, the hypothalamus and the forebrain. ②Following icv administration of AM(3 nmol/kg), double-labeled neurons for Fos and TH increased significantly in the area postrema(AP), the nucleus of the solitary tract(NTS), the nucleus paragigantocelluaris laterialis(PGL) and the locus coeruleus(LC). ③ Pretreatment with calcitonin gene-related peptide receptor antagonis CGRP_(8-37)(30 nmol/kg) significantly reduced the action of AM(3 nmol/kg) in the brain. Conclusion: AM activates the nuclei involved in cardiovascular regulation in the forebrain, the hypothalamus and the brainstem, and that the central actions of AM are induced by activating the catecholaminergic neurons of brainstem nuclei involved in cardiovascular regulation. CGRP receptor can mediate the effects of AM in brain.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2005年第2期146-149,i001,共5页
Chinese Journal of Applied Physiology
