摘要
目的研究HIV-1长期感染不进展现象与HIV-1 env基因变异之间关系.方法用PCR对11例感染HIV-1毒株10年以上者外周血单一核细胞样本进行扩增,获得env基因的核酸片段,对其C2～V3及邻区350～450个核苷酸序列及所属亚型进行分析,所得结果与HIV-1该亚型国际标准株进行比较,分析共享序列及突变序列,制作系统树,计算离散率.结果离散率和系统树分析11株毒株均为HIV-1 B亚型,基因离散率与国际标准株相比低于以往发现的陕西省流行株和文献报道的其他省份流行毒株.11株毒株V3环顶端四肽序列特征为GPGR的2例,占18.2%;为GRGQ的9例,占81.8%.在11株样本中均出现脯氨酸向异亮氨酸的变异.结论 env基因V3环顶端四肽GRGQ序列特征及脯氨酸向异亮氨酸的变异可能与HIV-1感染者长期感染而不发病的现象有关.
Objective To study the relationship between long-term HIV-1 infection phenomena and env gene mutation.Methods HIV-1 env gene was amplified by PCR from peripheral blood mononuclear cells (PBMCs) obtained from 11 HIV-1 long-term infectors from Shannxi.The C2-V3 region (350-450 bp) of them were sequenced and compared with common epidemic strains.Results Sequence analysis showed that these 11 strains all belonged to subtype B of HIV-1,gene divergence were much lower than those found in other provinces of China.They all had the mutation of aminoacid Pro turning to aminoacid Ile,and characteristic polypeptide GRGQ and GPGR occurred.Conclusion The mutation of aminoacid Pro turning to aminoacid Ile and characteristic polypeptide GRGQ at env gene may have some relationship with the phenomena of long-term HIV-1 infection.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2005年第5期257-260,共4页
The Chinese Journal of Dermatovenereology
基金
国家自然科学基金(编号:30300014)
陕西省科技攻关计划(编号:2003K10G9)
西安市工业科技攻关计划(编号:GG200467)。
