摘要
目的: 观察微载体悬浮培养系统(RCCS)对成人脂肪基质干细胞Adiposetissue derivedstromalcells(ADSCs)的培养效果,为大量、快速扩增组织工程种子细胞提供实验依据.方法: RCCS系统采用微载体Cytodex3为贴壁依赖性细胞ADSCs提供贴壁条件,浓度为5g/L.静止培养在12孔培养板中.两系统细胞接种密度均为1×108cells/L.分别观察细胞增殖情况,并对用RCCS培养的细胞进行细胞表型、分化能力进行检测.结果: 微载体悬浮培养9d后达到最大活细胞密度(1 60×109cells/L),静止培养第7日就达到最大活细胞密度(3. 38×108cells/L).在微载体悬浮培养条件下,ADSCs生长更为旺盛,细胞产量更高,优于静止培养. 11d后,ADSCs依然保持其干细胞特性.结论: 利用生物反映器以及微载体技术有利于ADSCs的大规模扩增,是扩增组织工程种子细胞的有效方法.
AIM: To explore the possibility of culturing Adipose tissue-derived stromal cells (ADSCs) in large quantity by microcarrier suspension system and the multidifferentiation ability of the cultured cells.METHODS: Adult human ADSCs cultured on microcarriers in spinner flasks were compared with those of statically cultured in 12 well plates.For the adherent growth of ADSCs,Cytodex 3,one kind of microcarries,was used at the concentration of 5 g/L.The cells were seeded in a density of 1×108/L in both cultural systems.RESULTS: After 9 days of cultivation,the results showed that a maximum viable cell density (1.60×109 cells/L) was reached in spinner cultured system,whereas the cell density increased to a maximum of 3.38×108 cells/L on the 7th day in static culture system.After spinner culture for 11 days,the ADSCs still maintained the characteristics of stem cells.CONCLUSION: Microcarrier culture system is an effective way to expand ADSCs as the seeding cells in tissues engineering.
出处
《第四军医大学学报》
CAS
北大核心
2005年第10期954-956,共3页
Journal of the Fourth Military Medical University
基金
国家"973"项目基金(G1999054308)
关键词
脂肪基质干细胞
细胞培养
微载体
组织工程
adipose stromal cells
cell culture
microcarrier
tissue engineering
