摘要
目的检测乙型病毒性肝炎病人外周血T细胞亚群和膜白介素-2受体(mIL—2R)表达水平并探讨其在乙肝发病机制中的作用。方法采用生物素-链霉亲和素法对188例乙肝病人外周血单个核细胞(PBMC)进行T细胞亚群及植物血凝素(PHA)诱导前后mIL—2R水平测定。结果乙肝病人T淋巴细胞亚群中CD3^+、CD4^+降低,CD8^+升高,CD4^+/CD8^+比值降低,外周血单个核细胞mIL—2R表达水平在PHA诱导前后均降低,与正常对照相比,差异有显著意义(P<0.01);另外,乙肝病人PBMC中HBV—DNA(+)病人与PBMC内HBV—DNA(-)病人相比,mIL—2R表达水平亦存在较大差异(P<0.01)。结论乙肝病人体内存在明显的细胞免疫功能紊乱,T细胞活化障碍,并与肝病的慢性化相关。
Objective To study the levels of T lymphocyte subsets and membrane interleukin-2 receptor (mIL-2R) on surface of peripheral blood mononuclear cells (PBMC) of the patients with hepatitis B and its role in the pathogenesis of hepatitis B. Methods The levels of T lymphocyte subsets and mIL-2R in PBMC before and after being stimulated with PHA were detected by biotin-streptavidin (BSA) technique in 188 cases with hepatitis B. Results In the patients with hepatitis B, the levels of CD3^+, CD4^+, and CD4^+/CD8^+were lower, but CD8^+ was higher than those in normal controls (P<0.01): and the total expression levels of mIL-2R in PBMC before and after being stimulated With PHA were also lower than those in normal controls (P<0.01). Of the patients with hepatitis B, the levels of T lymphocyte subsets and mIL-2R in PBMC with HBV-DNA (+) were lower than those with HBV-DNA (-), which were signicantly different (P<0.01). In addition, the levels of mIL- 2R in PBMC were lower in silence than those in inducting with PHA (P<0.01). Conclusions Both cellular and humoral immune functions are obviously disorder in patients with hepatitis B, which might closely associate with the chronicity of the patients with hepatitis B.
出处
《热带病与寄生虫学》
2003年第1期15-18,共4页
Journal of Tropical Diseases and Parasitology
