摘要
目的:研究黄连总碱(TAL)对小鼠结肠炎的作用及其机制。方法:实验设正常、模型、柳氮磺胺吡啶组(SASP,520mg/kg)和TAL组(150mg/kg)。正常组小鼠饮用蒸馏水,其余组自由饮用4%葡聚糖硫酸钠(DSS)水溶液,同时分别灌胃给予溶剂或干预药物(0.2ml/10gwt,1次/d×7d)。记录小鼠疾病活动指数(DAI);测定结肠组织MDA含量,SOD、MPO活性及ICAM鄄1、NF鄄κBp65表达水平。结果:模型组DAI显著增高,结肠粘膜损伤严重;MDA含量、MPO活性及ICAM鄄1和NF鄄κBp65表达明显升高,SOD活性下降(P<0.01)。SASP520mg/kg能明显逆转上述改变;TAL150mg/kg具有相似的作用。结论:TAL可能通过抗氧自由基作用,抑制炎性细胞活化、迁移及NF鄄κB激活,缓解小鼠结肠炎症。
Objective: To investigate the effect of total alkaloids from corptis root (TAL) on experimental ulcerative colitis (EUC) of mice. Methods: The colitis model was established allowing the mice drinking 4% dextran sulfate sodium (DSS) solution freely for 7 days, and during the same period, either salicylazosulfapyridine (SASP, 520 mg/kg) or TAL (150 mg/kg), and solvent (0.2 ml/10 mg wt) were given once a day, respectively. At the same time, normal mice drank distilled water freely and were given solvent (0.2 ml/10 mg wt). EUC was evaluated by disease activity index (DAI), myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, malondialdehyde (MDA) content, as well as the expressions of NF-κB p65 and intercellular adhesion molecule 1(ICAM-1) proteins. Results: DAI, MPO activity, MDA content and expressions of ICAM-1 and NF-κB in DSS-induced mice were markedly increased, while SOD activity decreased (P < 0.01), compared with that of the healthy control. Treatment of SASP 520 mg/kg reversed the events above; TAL 150 mg/kg had the similar effects on SASP in DSS-induced mice. Conclusion: TAL has protective effects on DSS colitis. The mechanism possibly contributes to their antioxidant function and anti-inflammatory activity associated with inhibiting the NF-κB activation and the inflammatory cells infiltration.
出处
《儿科药学杂志》
CAS
2005年第3期7-9,12,共4页
Journal of Pediatric Pharmacy
关键词
葡聚糖硫酸钠
黄连总碱
结肠炎
Dextran sulfate sodium (DSS)
Total alkaloids from corptis root (TAL)
Colitis
