摘要
目的 观察全血标本冰箱长时间保存对血细胞计数及白细胞(WBC)分类结果 的影响.方法 把50例标本在4℃保存,连续5 d用Coulter LH750测定结果,用SPSS11.0做统计学分析.结果 WBC、血小板(PLT)计数可以稳定3 d,红细胞(RBC)、血红蛋白(HGB)、平均红细胞血红蛋白含量(MCH)、平均红细胞体积(MCV)、红细胞分布宽度(RDW)、红细胞压积(HCT)至少可稳定5 d,平均红细胞血红蛋白浓度(MCHC)、PDW、平均血小板体积(MPV)1 d后与即刻比较差异有显著性(P＜0.01).WBC分类结果 从1～2 d开始,与即刻比较差异有显著性(P＜0.01),同时仪器出现大量的报警提示.结论 全血标本4℃保存,提高了部分血细胞计数结果 的可靠性,但不能保证WBC分类结果 .报告时应注明'标本放置时间过长,对部分结果有影响'.
Objective To observe changes of results in automated complete blood cell count and differential leukocyte count by prolonged storage of blood sample at 4 (°C) refrigerator. Methods 50 blood specimens were stored at 4 (°C) refrigerator, and determined by Coulter LH750 for 5 days consecutively, results were analysed by SPSS 11.0. Results WBC,PLT were stable for 3 days while RBC, HGB, MCH, MCV, RDW, HCT were stable for at least 5 days; MCHC, PDW, MPV had significant difference with instant results from day of 1 onward; differential leukocyte count results had significant difference with instant results from days of 1-2 onwards, at the time Coulter LH750 suspected frequently. Conclusions Store complete blood at 4 (°C) refrigerator can improve the reliability of part results but not so with differential leukocyte count. For reporting result of prolonged specimen, comments such as “specimen long time stored, part of results may be affected” should be suspended.
出处
《检验医学》
CAS
北大核心
2005年第3期195-197,共3页
Laboratory Medicine
