摘要
目的:比较量效最佳条件下给予纳洛酮与加兰他敏对东莨菪碱所致大鼠空间工作记忆障碍的作用。方法:实验于2003-04在汕头大学医学院动物实验中心完成。28只大鼠随机分成4组,每组7只。正常对照组、东莨菪碱组、纳洛酮治疗组(东莨菪碱+纳洛酮)和加兰他敏治疗组(东莨菪碱+加兰他敏)。东莨菪碱以0.4mg/kg、纳洛酮以3mg/kg、加兰他敏以2mg/kg给药(前3组给药剂量均为最佳量效关系剂量),正常对照组予生理盐水2.0mL,均在实验前30～40min腹腔给药。采用水迷宫延缓性匹配作业,开始进行空间工作记忆训练(隐匿平台任意放置池中某一位置,大鼠在任一位置面壁放入水池,其逃避潜伏期仍以60s为限,此过程为实验1,找到平台后,允许其逗留10s,作为工作记忆,然后将其再从同一位置面壁放入水池,大鼠会利用工作记忆第二次找到平台,此过程为实验2)。记录比较每只大鼠两次实验逃避潜伏期的时间,评估不同药物对大鼠空间工作记忆能力的影响。结果:各实验组大鼠无脱失,均纳入分析。在两次实验逃避潜伏期比较中:正常组实验2较实验1减少[(13.4±8.2),(46.4±17.7)s,P<0.01];纳洛酮治疗组实验2较实验1减少[(9.0±3.8),(27.9±14.3)s,P<0.05];东莨菪碱组和加兰他敏治疗组实验2较实验1变化不大[(18.1±9.8),(23.6±14.3)s;(12.0±9.0),(25.4?
AIM:To compare the effects of naloxone and galanthamine on scopolamine induce d impairment of spatial working memory in rats in the best dose effect manner. METHODS:The experiment was carried out in the animal experimental center,Medic al College of Shantou University in April 2004.Twenty eight rats were randomly divided into 4 groups with 7 rats in each group:normal control group, scopolamin e group,naloxone treated group(scopolamine+naloxone),galanthamine treated group (scopolamine+galanthamine).Scopolamine(0.4 mg/kg),naloxone(3 mg/kg) and galanth amine(2 mg/kg) were given correspondingly(the administrating doses in the first 3 groups were of the best dose effect relationship),saline(2.0 mL) was given to rats in the normal control group,all the rats were administrated intraperitonea lly 30 to 40 minutes before experiment.A delayed matching to sample variant of Morris water maze was adapted,and then they received training of spatial workin g memory(Experiment 1:A hidden platform was placed randomly in the pool,and then a rat was put in any place facing to the wall of pool,and the escape latency wa s limited to 60 s;Experiment 2:The rat was allowed to have an interval of 10 s a s the working memory after they found the platform,and then the rat was put into the pool in the same place facing to the wall,and the rat would find the platfo rm for the second time by using working memory).The escape latencies of each rat in the two experiments were recorded and compared,and the effects of different drugs on the spatial working memory ability of the rats were assessed. RESULTS:All the rats were involved in the analysis in each group without delet ion.In comparison of the escape latency in the two experiments, it was decreased in experiment 2 as compared in experiment 1 in the normal control group[(46.4± 17.7),(13.4±8.2) s,P< 0.01] and naloxone treated group[(27.9±14.3),(9.0±3.8) s,P< 0.05],but there were no obvious changes in the scopolamine group[(23.6±14. 3),(18.1±9.8) s,P >0.05] and galanthamine treated group[(25.4±24.3),(12.0±9.0 ) s, P >0.05]. CONCLUSION:Under the condition of the best dose effect,scopolamine can impair the spatial working memory of rats,and naloxone can ameliorate this impairment, but galanthamine has no effect in improving this impairment.
出处
《中国临床康复》
CSCD
北大核心
2005年第16期110-111,共2页
Chinese Journal of Clinical Rehabilitation
基金
广东省自然科学基金资助项目(000825)~~
