摘要
目的:研究解郁丸及其拆方对抑郁模型小鼠的抗抑郁作用及其差异比较。方法:实验在北京大学医学部基础医学院药理系神经精神药理实验室进行。实验采用ICR雄性小鼠,体质量18～25g,随机分成8组:对照组(5g/L羧甲基纤维素钠)、氟西汀组(20mg/kg)、解郁丸低剂量组(65.75mg/kg)、解郁丸中剂量组(131.5mg/kg)、解郁丸高剂量组(263mg/kg)、逍遥散组(52.34mg/kg)、郁百合方组(14mg/kg)、甘麦大枣方组(21.6mg/kg)。采用小鼠强迫游泳实验、悬尾实验两个行为绝望实验观察解郁丸及其拆方的抗抑郁作用,为了鉴别上述各药是否具有中枢兴奋性的作用,又同时测定小鼠的自主活动,以确定解郁丸等抗抑郁药的可靠性。结果:强迫游泳实验、悬尾实验显示氟西汀组及解郁丸中、高剂量组、郁百合方、逍遥散、甘麦大枣方各组均可显著缩短小鼠不动时间(P<0.01)。解郁丸中、高剂量组、郁百合方、逍遥散、甘麦大枣方各组与氟西汀组比较无明显差异(P>0.05)。解郁丸中、高剂量组与郁百合方、逍遥散、甘麦大枣方各拆方组比较无明显差异(P>0.05)。小鼠的自主活动显示解郁丸低、中、高剂量组和郁百合方、逍遥散、甘麦大枣方各拆方组对小鼠自主活动均无显著影响(P>0.05)。结论:解郁丸及其拆方各组均可以改善小鼠抑郁状态行为,具有抗抑郁作用,且无?
AIM:To compare the difference of the antidepressant effect between Jieyuwan an d its components on depressive mouse models. METHODS: The experiment was carried out in the pharmacological laboratory for neurology and psychology of the Department of Pharmacology,School of Basic Medic al Sciences,Peking University Health Science Center.Male ICR mice were randomly divided into 8 groups:control group(5 g/L carboxymethylcellulose sodium),fluoxet ine group (20 mg/kg),Jieyuwan small,middle and large dose groups (65.75,131.5 an d 263 mg/kg),Xiaoyaosan group (52.34 mg/kg),Yubaihe Fang group(14 mg/kg) and Gan mai Dazao Fang group(21.6 mg/kg).The antidepressant effects of Jieyuwan and its components were observed with two behavioral despair experiments of mice forced swimming test and mice tail suspension test.In order to identify whether the abo ve mentioned drugs had the role in central excitability,the automatic activitie s of the mice were determined to make sure the reliability of Jieyuwan and other antidepressants. RESULTS:The forced swimming test and tail suspension test showed that the immo bility time of rats was significantly shortened in the fluoxetine group,Jieyuwan middle and large dose groups,Yubaihe Fang group, Xiaoyaosan group and Ganmai Da zao Fang group(P< 0.01).Compared with the fluoxetine group, there was on obvious difference in the Jieyuwan middle and large dose groups,Yubaihe Fang group,Xiao yaosan group and Ganmai Dazao Fang group(P >0.05).The immobility times in the Ji eyuwan middle and large dose groups were not obviously different from those in t he Yubaihe Fang group,Xiaoyaosan group and Ganmai Dazao Fang group(P >0.05).The automatic activities of the mice showed that their automatic activities were not notably affected by small,middle and large dose Jieyuwan,Yubaihe Fang, Xiaoyaos an and Ganmai Dazao Fang(P >0.05). CONCLUSION:Jieyuwan and its components can ameliorate the depressive behaviors of mice.It has the antidepressant effect but has no effect of central excitabil ity.The antidepressant effect of Jieyuwan is not different from those of its components.
出处
《中国临床康复》
CSCD
北大核心
2005年第16期115-117,共3页
Chinese Journal of Clinical Rehabilitation
