摘要
从微小体积鼻咽癌活检标本中获取纯净癌细胞一直是鼻咽癌分子生物学研究中的难题.为了寻找一种能从鼻咽癌活检组织中获得高纯度、高质量RNA来完成cDNA微阵列(cDNA Microarray)实验的简便实用方法,采用RNAlater技术保存鼻咽癌活检组织,显微切割技术来获得高纯度鼻咽癌细胞,利用RNA线性扩增技术得到cDNA微阵列实验所需RNA.结果表明:利用RNAlater技术可以很好地保持组织RNA的稳定,通过优化显微切割和RNA线性扩增的条件获得了cDNA微阵列实验所需的高纯度、高质量RNA.
It is a difficult problem in molecular biology study of nasopharyngeal carcinoma (NPC) to obtain pureness tumor cells from tiny NPC tissue. To seek a convenient and applied way to obtain pureness and high quality RNA from NPC tissue for cDNA Microarray, the study was designed to use RNA later technology to keep the NPC dissected tissue and obtain pureness NPC cells by microdissection and gain RNA through RNA line amplification technology. The results hinted that RNAlater technology could keep the stability of NPC tissue and obtain the pureness and high quality RNA for cDNA Microarray combined with microdissection and RNA line amplification through optimum conditions.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2005年第5期463-467,共5页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目(30300201
30470955
3030560
30200312)
新世纪优秀人才支持计划资助项目(NCET-04-0761)~~
