摘要
目的探讨胃癌Hsp70-肽复合物致敏树突状细胞(DC)抗肿瘤作用。方法采用GM-CSF,IL-4刺激,培养胃癌患者外周血单个核细胞,增殖产生大量DC,用离子交换层析法从临床胃癌组织中提取Hsp70肽复合物,并行westernbolt鉴定,Hsp70-肽复合物修饰DC,通过细胞因子检测试剂盒对修饰DC上清液进行检测,用DC激活混合T淋巴细胞,观察其对胃癌细胞的杀伤作用。用未被Hsp70-肽复合物修饰DC作对照。结果Hsp70-肽复合物DC分泌IL-12、TNF-α、IL-1β含量分别为(305.02±20.70)pg/ml、(480.61±32.52)pg/ml和(519.60±39.12)pg/ml,而未被Hsp70修饰DC分泌IL-12、TNF-α、IL-1β的含量分别为(3.81±0.84)pg/ml、(3.50±0.21)pg/ml、(50.11±2.86)pg/ml,两者比较,有显著性差异(P<0.01)。Hsp70修饰DC激活的T淋巴细胞后对胃癌细胞的杀伤率为(93.54±8.26)%明显高于对照组的(6.20±0.38)%(P<0.01)。结论胃癌Hsp70-肽复合物致敏DC并激活T淋巴细胞后对胃癌细胞具有很强的杀伤作用。
Objective To study antitumor effect of DC modified by stomach neophasma Hsp70-petide complexes.Methods Peripheral blood PBMC of stomach cancer were cultured with GM-CSF and Il-4 stimulating,thus proliferated to produce plenty of DC.Extract Hsp70-tumor peptide complexes from stomach nephasms with chromatography of protein thus comfirmed by western-blot.Afer DC were modified by Hsp70tumor peptide complexes,DC supernatant was detected using cytokine detected kit.T lymphocytes was stimulated and their ability of killed tumor cells was investigated.Results DC modified by stomach neophasmsHsp70-peptide complexes plentifully secret IL-12、TNF-α、IL-1β.Their content was (305.02±20.70 )pg/ml、(480.61±32.52)pg/ml、(519.60±39.12)pg/ml, respectively.The contents of IL-12、TNF-α、IL-1β secreted by unmodified Dc was(3.81±0.84)pg/ml、(3.50±0.21)pg/ml、(50.11±2.86)pg/ml with an obvious difference(P<0.01).The T lymphocytes induced with modified DC had a strong killing role to tumor cells,killing rate was (93.5±8.2)% and their difference was obvious compared with that [LM]in control group[(6.20±0.38)%,P<0.01].Conclusion Stomach neophasma Hsp70-petide complexes can modify DC and the T lymphocytes induced by modified DC could kill tumor cells.
出处
《江西医学院学报》
2005年第3期1-3,6,共4页
Acta Academiae Medicinae Jiangxi
基金
江西省自然科学基金资助项目(0140061)
