摘要
目的探讨乌头类生物碱(四逆汤煎剂)对体外循环心肌CuZnSOD基因表达的影响。方法28例成年瓣膜置换患者随机分成对照组14例和乌头类生物碱口服(四逆汤煎剂)组14例。术前1周乌头类生物碱组依照中医辩证给予乌头类生物碱每天1剂口服,术后分别取右心耳组织测心肌组织铜锌超氧歧化酶CuZnSODmRNA含量。提取各组心肌组织总RNA通过逆转录聚合酶链反应(RTPCR),以βactin为内参照对CuZnSOD基因的表达水平进行检测。结果乌头类生物碱(四逆汤煎剂)组(1.310±0.135)CuZnSOD基因表达显著强于对照组(1.060±0.183)。结论乌头类生物碱能上调CuZnSOD基因的表达,发挥对体外循环心肌缺血再灌注损伤的保护作用。
Objective To detect the effect of aconitine akaloid akaloid (Sini decoction) on Cu-ZnSOD expression in the ischemic myocardium of extracorporeal circulation.Methods Twenty-eight cardiac valve replacement patients in adult were divided into control and aconitine akaloid akaloid groups.Preoperation the aconitine akaloid group was treated with aconitine akaloid (SND) for 7 days once every day.Total RNA was extracted from the myocardium in each group.The effect of aconitine akaloid (Sini decoction) on the expression of Cu-ZnSOD gene was detected by RT-PCR.Results The expression of Cu-ZnSOD mRNA in aconitine akaloid (Sini decoction) group (1.310± 0.135) was significantly up-regulated as compared with the control group (1.060± 0.183).Conclusion Aconitine akaloid (Sini decoction) can promote the expression of Cu-ZnSOD gene,which may be related to its protective effect on ischemic myocardium.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第7期782-783,共2页
Chinese Journal of Experimental Surgery
基金
广东省科委资助项目(99M04602G)
