摘要
以无刺红、丰满红、黄树莓3个树莓品种为试材,取其茎尖和茎段为外植体,进行了离体培养与植株再生频率的研究.结果表明:(1)芽分化阶段:无刺红在MS+6-BA 0.5 mg/L加活性炭0.1%的培养基上表现最优,芽的诱导率最高,为95.24%;黄树莓最佳培养基为MS+KT0.5 mg/L+NAA 0.03 mg/L+GA 6 mg/L加活性炭0.1%,芽的诱导率为94.44%;丰满红最优培养基为MS+KT 0.5 mg/L加活性炭0.1%,芽的诱导率为88.89%.(2)继代培养阶段:无刺红和丰满红的培养基均以MS+6-BA 1.0 mg/L+NAA 0.1 mg/L为最优,增殖倍数分别为7倍和5倍;黄树莓最佳培养基为MS+6-BA 1.0 mg/L+IBA 0.1 mg/L,增殖倍数可达5倍.(3)生根培养阶段:无刺红以1/2 MS+IBA 0.3 mg/L+IAA 0.5 mg/L培养基为佳,生根率可达93.33%;黄树莓以1/2 MS+IBA 0.6 mg/L+IAA 1.0 mg/L培养基为佳,生根率可达86.67%,丰满红以1/2 MS+IBA 1.0 mg/L+NAA 1.0 mg/L培养基为佳,根诱导率为86.67%.在两步移栽法中,河沙苗盘中的炼苗成活率分别都达到90%以上,移栽到腐殖土+河沙(3∶1)基质上的成活率也都达96%以上.
The stem segments and tips of red raspberry,'Fengmanhong' and yellowberry were used as explants to study their isolated culture and plant regeneration frequency in vitro culture.The appropriate media for bud differentiation was MS+6-BA 0.5 mg/L+ active carbon (0.1%) which was suitable for 'thornless red raspberry' and the induced rate of buds was the highest and up to 95.24%; MS+KT 0.5 mg/L+NAA 0.03 mg/L+GA 6 mg/L+ active carbon 0.1% was suitable for 'Yellowberry' and the induced rate was up to (94.44%); MS+KT 0.5 mg/L which appended carbon 0.1% was suitable for 'Fengmanhong' and the induced rate was up to 88.89%. For multiplication, MS+6-BA 1.0 mg/L+NAA 0.1 mg/L was suitable for 'thornless red raspberry' and 'Fengmanhong', the multiple for multiplication was up to 7 and 5; MS+6-BA 1.0 mg/L+IBA 0.1 mg/L was suitable for yellowberry, the multiple for multiplication was up to 5. For rooting,1/2 MS+IBA 0.3 mg/L+IAA 0.5 mg/L was suitable for 'thornless red raspberry' and the rate of rooting was 93.33%; 1/2 MS+IBA 0.6 mg/L+IAA 1.0 mg/L was suitable for 'yellowberry' and the rate of rooting was 86.67%;, 1/2 MS+IBA 1.0 mg/L+NAA 1.0 mg/L was suitable for 'Fengmanhong' and the rate of rooting was (86.67%). The survival rates of seedlings of all varieties in river sand were more than 90%.If transplanted to humus :river sand=3∶1, the survival rates of three varieties were more than 96%.
出处
《延边大学农学学报》
2005年第2期126-131,共6页
Agricultural Science Journal of Yanbian University
关键词
树莓
培养基
离体培养
激素
再生频率
raspberry
media
vitro culture
hormone
regeneration frequency
