摘要
在0.40 mol/L的HAc-NaAc (pH4.9)缓冲液中,利福平在碳糊电极(CPE)上有一灵敏的吸附氧化峰,峰电位为0.86 V(vs.SCE).该氧化峰的二阶导数峰电流与利福平的浓度在1.0×10-8～2.0×10-6 mol/L (富集60 s) 范围内成良好的线性关系,相关系数为0.998,检出限为5.0×10-9 mol/L(S/N=3,富集90 s),探讨了利福平在碳糊电极上的伏安性质和电极反应机理,并且成功应用于人体尿液中利福平含量的测定.
A highly selective and rapid new anodic adsorptive voltammetric method has been developed for the determination of ultra trace amount of Rifampicini at a carbon paste electrode (CPE) in the current paper.The Rifampicini is adsorbed on the surface of the CPE in 0.40 mol/L HAc-NaAc buffer solution (pH4.9) yielding one oxidation peak at 0.86V (vs.SCE). The relationship between the peak current and Rifampicini concentration is linear in the range of 1.0 10-8～2.0×10-6 mol/L with the correlation coefficients 0.998. The detection limit is 5.0×10-9 mol/L (S/N=3) for 90 s acummulation. The electrode reaction mechanism of Rifampicini was investigated. The proposed method was applied to directdetermine trace Rifampicini in the urine sample in good agreement with the results of the spectrophometric determination.
出处
《湘潭大学自然科学学报》
CAS
CSCD
北大核心
2005年第2期108-111,共4页
Natural Science Journal of Xiangtan University
基金
长春市应用化学研究所电分析化学国家重点实验室基金项目(SKLEAC2003-5)
关键词
利福平
阳极吸附伏安法
碳糊电极
Rifampicini
Adsorptive anodic-stripping voltammetry
Carbon paste electrode
