摘要
样品溶于水,在酸性条件下,以次氯酸钠柱前衍生。在C18柱上用乙腈:水(体积比为70:30)为流动相,在314nm处测定,外标法反相高效液相色谱法快速测定食品中的甜蜜素,加标回收率为96.1%-101.8%之间,食品中共存的苯甲酸、山梨酸、安赛蜜、色素等不影响测定,方法简捷、准确。
A rapid determination of cyclamate in food by the reversed phase high performance liquid chromatography with. NaClO as a pre-column derivation reagent at pH<7 on a C18-column eluted in 10min with a mobile phase of CH2CN: H2O mixture(70 : 30), and a photometric detector at 314nm. The recoveries are in the range of 96.1%-101. 8% without interferences of benzoic, sorbic, acesulfame K,color. This method is simple,rapid,accurate and repeatable.
出处
《光谱实验室》
CAS
CSCD
2005年第3期583-585,共3页
Chinese Journal of Spectroscopy Laboratory
