摘要
目的:研究大鼠肠上皮细胞(IEC)缺血缺氧后基因表达谱的改变,寻找与IEC损伤相关的基因.方法:建立缺血缺氧大鼠IEC的实验模型,实验分为对照组、缺血组、缺氧组和缺血缺氧组.荧光逆转录标记mRNA,采用大鼠基因表达谱芯片检测正常IEC与缺血组、缺氧组及缺血缺氧组IEC基因表达谱,并对比分析检测结果.结果:与正常IEC相比,缺血组基因表达有差异的共207组,其中132组下调,75组上调;缺氧组基因表达有差异的共168组,其中84组下调,84组上调;缺血缺氧组基因表达有差异的共321组,其中97组下调,224组上调.结论:应用基因芯片技术筛选了与IEC缺血缺氧损伤密切相关的差异表达基因,为阐明这方面的机制提供了新的线索.
AIM: To investigate the change of gene expression in intestinal epithelial cells (IECs) under ischemia and anoxia in rats and to search for the correlated genes with IECs injury. METHODS: lECs injury model was induced by ischemia and anoxia, and the cells were divided into four groups: control group, ischemia group, anoxia group and ischemia and anoxia (I/A) group. Fluorescence reverse transcription was used to label mRNA. The cDNA microarray was used to detect the difference between gene expression of control group and experiment groups. RESULTS: There were 207 genes differently expressed between control and ischemia group, of which 132 were down-regulated while 75 were up-regulated. One hundred and sixty-eight genes were differently expressed between control and anoxia group, of which 84 were down-regulated while 84 were up-regulated. Between control and I/A group, 321 genes were differently expressed, of which 97 were down-regulated while 224 were up-regulated. CONCLUSION: cDNA microarray can be used to screen diversified gene expression related to injury under ischemia and anoxia, which brings some new clues for studying the mechanism of IECs injury.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第11期1263-1266,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30271286上海市科委青年科技启明星跟踪计划资助项目
No.02QMB1406~~
