摘要
铝毒害是限制酸性土壤中植物生长的重要因素,以荞麦(FagopyrumesculentumMoench.)为实验材料,在沙培条件下,用不同浓度(0,10,100,1000mg/L)、不同pH(3,4,5)的铝溶液处理,在出苗后的第14d和28d测试与铝胁迫有直接关系的5个生理指标。结果表明,溶液的铝浓度和pH的变化对荞麦生理都有明显的影响,最低浓度的铝处理10mg/L在不同pH下已经影响了荞麦的正常生长,和对照相比根系活力(RA)下降、根质膜透性(MP)增大、叶片内丙二醛(MDA)和游离脯氨酸(Pro)的含量以及过氧化物酶(POD)的活性升高,并且随着铝处理浓度的升高,荞麦体内各生理指标和对照差异性加大;同一铝浓度下pH5和pH3相比,MP降低、RA增强、叶片内MDA和Pro的含量以及POD的活性有所下降。铝胁迫下荞麦体内各生理指标的变化有显著的相关性。随着荞麦的生长,铝毒害有所缓解。
Aluminum toxicity has been a serious agricultural problem in acid soils, so the study of physiological response of plants to aluminum stress is of great significance. In this research, five physiological indices of buckwheat in acid-aluminum stress were investigated. Buckwheat was cultured in sand which was treated by different concentrations of aluminum (0,10,100,1 000 mg/L) and pH value(3,4,5). At 14d and 28d after buckwheat sprouting, roots and leaves were used to probe into the changes of physiological characteristics. The result indicated that under acid-aluminum stress, even in10mg/L pH5, the cells of both root and leaf were damaged and the growth of buckwheat was influenced. With the rise of aluminum concentration or with the drop of pH, the root system activity (RA) was decreased, the membrane permeability of root cells (MP),the amount of malonaldehyde (MDA) and free proline (Pro) in leaves were increased, and the activity of peroxidase (POD) was raised. Especially 1 000 mg/L pH3, the cells of buckwheat were destroyed worst. The membrane permeability of root cells was 2.59 times and the amount of malonaldehyde in leaves was 3.78 times than the control at 14d after sprouting, 2.10 times and 1.95 times at 28 d. These physiological indices of buckwheat had significant correlation with each other under acid-aluminum stress. Aluminum toxicity became less in its growth.
出处
《水土保持学报》
CSCD
北大核心
2005年第3期105-109,共5页
Journal of Soil and Water Conservation
基金
浙江省自然科学基金(303461
Y504135)
浙江省分析测试基金(03091)资助项目
关键词
荞麦
酸铝胁迫
生理响应
buckwheat
acid-aluminum stress
physiological response
