摘要
目的 在大肠杆菌中表达可溶性EC -SOD。方法 将表达质粒EC -pet2 8a(+)转入宿主菌BL2 1 (DE3)plysS中进行表达,在低温下培养含表达质粒EC -pet2 8a(+)的宿主菌BL2 1 (DE3) ,通过SDS -PAGE电泳,观察其在超声上清中的表达。结果 EC -SOD在BL2 1 (DE3)plysS中表达,其量比在BL2 1 (DE3)降低约2 0 %,上清中有明显表达;在2 0℃和1 5℃培养时,EC -SOD在BL2 1 (DE3)的超声上清中有表达。结论 EC -SOD在BL2 1 (DE3)plysS中或在BL2 1 (DE3)中低温培养,均可实现部分上清中的表达。
Objective To express the resolution EC-SOD by E.coli.Methods The expression plasmid EC-pet28a(+) was transformed into BL21(DE3)plysS and BL21(DE3)containning expression plasmid EC-pet28a(+) was cultivated in low temperature. The expression of EC-SOD in supernatant was observed by SDS-PAGE. Results The expressed amount of EC-SOD by BL21(DE3)plysS was less about 30% than that by BL21(DE3), and it could express in supernatant obviously; EC-SOD could also express in supernatant by BL21(DE3)which was fermented in the temperature 20℃ and 15℃.Conclusion EC-SOD can express in supernatant by BL21(DE3)plysS or by BL21(DE3) fermented in low temperature.
出处
《食品与药品》
CAS
2005年第04A期31-34,共4页
Food and Drug
