摘要
用地高辛（Ｄｉｇ）标记原位杂交技术，对正常子宫颈上皮ＮＣｘ，人乳头瘤病毒１６（ＨＰＶ１６）感染的宫颈癌上皮ＳｉＨａ及宫颈湿疣上皮Ｗ１２进行了肿瘤坏死因子α（ＴＮＦα）ｍＲＮＡ表达的研究。在ＳｉＨａ及Ｗ１２细胞中均可见ＴＮＦαｍＲＮＡ的表达，ＮＣｘ中未见其表达。结果提示：宫颈ＨＰＶ感染对细胞内ＴＮＦαｍＲＮＡ表达有促进使用，且上皮的组织学分化对其表达亦有影响。
The expression of tumor necrosis factor-α(TNFα)mRNA in normal cervical keratinocytes(NCx),human papillomavirus 16(HPV16)-infected keratinocytes SiHa derived from cervical carcinoma and W12 from cervical warts,was detected by digoxigenin labelled in situ hybridization.TNFα mRNA was found both on SiHa and W12,but not on NCx.This indicates that HPV infection in cervix enhances the expression of TNFα-mRNA and the degree of differentiation of the keratinocytes also influenced the expression.
出处
《青岛医学院学报》
1994年第2期91-93,F002,共4页
Acta Academiae Medicinae Qingdao Universitatis
