摘要
目的:建立以光谱法鉴定黑海参中多糖成分的方法。方法:采用电泳法测定黑海参中多糖纯度,并用红外分光光度法、紫外分光光度法、核磁共振波谱法对其红外吸收光谱、紫外吸收光谱、核磁共振碳谱、核磁共振氢谱进行结构鉴定。结果:电泳后凝胶带呈蓝色,光谱检测结果可相互解释。结论:黑海参中存在均一组分的多糖成分,且为一种含硫酸酯基的酸性多糖,但不含糖肽;α-构型,存在α-吡喃葡萄糖,分子中的羟基形成分子内氢键。
OBJECTIVE:To establish the method to identify the polysaccharide element in Holothuria atra Jeager by spectroscopy.METHODS:The purity of polysaccharide in Holothuria atra Jeager was determined by electrophoretic method;the infrared absorption spectrum,ultraviolet absorption spectrum,carbon spectra and hydrogen spectra of NMR were identified in structure by infrared spectrophotometry,ultraviolet spectrophotometry and NMR spectrometry respectively.RESULTS:The gel band after electrophoresis took blue color and the results of spectrum determination accountable for each other.CONCLUSION:Uniformly constituent of polysaccharose was found in the Holothuria atra Jeager,which was an acidic polysaccharose with vitriolic esterfunction,without glucopeptide;α-configuration,α-glucopyranose was found and the intramolecular hydrogen bond was formed by hydroxide radical.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第13期1012-1014,共3页
China Pharmacy
基金
国家自然科学基金项目(30271677
30371837)
关键词
黑海参
光谱
鉴定
多糖成分
Holothuria atra Jeager
Spectrosopy
Identification
Polysaccharide
