摘要
用ｐ５３、ｐ２１、ｐ１８５，通过免疫金银染色方法，对７８例大肠癌、１４例大肠息肉进行了检测。结果显示：大肠癌ｐ５３、ｐ２１、ｐ１８５的阳性率分别为５３．９％、４６．２％、５１．３％；大肠息肉仅有１４．３％｛２／１４）显示ｐ１８５弱阳性。二者之间有显著性差异。大肠癌旁正常粘膜上皮阳性卒ｐ５３为０、ｐ２１２．６％、ｐ１８５５．１％，与其肿瘤区比较Ｐ＜０．０１；７８例大肠癌中，有３３例（４２．３％）ｐ５３与ｐ２１同为阳性，ｐ５３在大肠低分化及粘液腺癌中的阳性表达明显高于高、中分化腺癌（ｐ＜０．０１）。
eventy eight cases of carcinoma and 14 cases of poly of large intestine were examined to observe the expression of p53,rasp21 and p 185 oncoproteins with immunogold silver staining method.The positive expressions for p53,rasp21 and p185 wcre 53.9%, 46.2% and 51.3% respectively.But only poorly posi-tive expression was found in large intestine polyps for p 185 staining.The difference between the carcinomas and polyps for p185 staining was significant(P<0.01).The noimal mucoepithelium near the cancer region expressed positive staining for p53, rasp21 and p185 were 0%,2.6% and 5.1 % respectively These results were also significantly different when compared to cancer tissue (P<0.01).In addition,of these 78 cases, 33 cases(42.3%) gave coordinately positive expression for p53 and rasp21 oncoprotein;p53 positive expression was obviously stronger in poorIy differentiated carcinoma and mucoadenocareinoma than in highly and moderately differentiated adenocarcinoma(P<0.01)
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1994年第2期97-99,T027,共4页
Chinese Journal of Pathology
