摘要
本研究采用出生２４小时内的新生兔，从其四肢长骨中分离出破骨细胞，与盖玻片、骨磨片共同培养。相差显微镜观察到分离的多核细胞能够移动，并在骨片上形成吸收陷窝．扫描电镜观察到这些吸收陷窝内的胶原原纤维．另外，这些细胞用目前公认的鉴定破骨细胞的标志─—酸性磷酸酶和降钙素染色，呈阳性反应。这些结果均表明：分离、培养破骨细胞的实验技术是成功的．
Abstract Osteoclasts were isolated rom one-day-oldneonatal rabbit’s long bones and cultutred on glass orbone slices.Under phasecontrast microscope,it hasbeen found that, these isolated multinucleated giautcells can migrate and generate resorption lacunae onbone slices. Scanning electron microscopy showedthat such areas appeared as excavated areas linedwith fibrillar net work.In addition.these cellsstained positively for acid phosphatase and calcitoninwhich are matkers for osteoclasts. These lesultssuggested that the procedure of isolation of osteo-clasts from the long bone of the neonatal rabbitswas successful.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1994年第1期43-46,T001,共5页
Chinese Journal of Orthopaedics
