摘要
目的建立并分析小鼠隐孢子虫卵囊的形态学参数,明确小鼠隐孢子虫卵囊的形态学变化特点。方法昆明株小鼠经地塞米松免疫抑制10d后,取粪便涂片、改良抗酸染色法检测小鼠隐孢子虫卵囊。应用摄像系统采集1190个小鼠隐孢子虫卵囊的图像,利用计算机数字图像分析系统测定卵囊的长径(L)、横径(W)、周长、面积和等效直径。用SPSS数据处理软件(V11.0)分析测定各组数据,计算其卵形指数。结果1190个小鼠隐孢子虫卵囊长径均值为5.93μm,95%可信区间为3.31～8.51μm;卵囊横径的均值为4.96μm,95%可信区间为3.26～6.66μm;周长均值为18.03μm;面积的均值为16.08μm2;卵形指数(L/W)为1.19。结论利用计算机显微图像处理系统所获得的小鼠隐孢子虫卵囊形态学参数数据客观、精确,为量化及评价小鼠隐孢子虫卵囊的形态奠定了基础。
Objective To establish and analyze the morphological parameters of the oocysts of Cryptosporidium muris for defining their morphological change. Methods Oocysts were collected from KM mice(immunodepressed by dexamethasone for 10 days) and examined with modified acid-fast staining. Images of 1 190 oocysts were acquired by photograph system. The length, width, perimeter, area and equivalent diameter of the oocysts were obtained by computer digital image processing system and analyzed by SPSS software (Version 11.0). Result The average length of the oocysts was 5.93 μm, ranging from 3.36 μm to 8.51 μm in 95% confidence interval of them. The average width was 4.96 μm, ranging from 3.26 μm to 6.66 μm in 95% confidence interval of them. The average perimeter was 18.03 μm and the average area was 16.08 μm2. Conclusion Data obtained from the computer system are objective and precise, offering scientific foundation for measuring the oocysts and for identifying Cryptosporidium spp.[
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2005年第3期175-177,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
辽宁省教育厅资助项目(No.202013143)~~
关键词
小鼠隐孢子虫
卵囊
数字图像处理
形态学参数
Cryptosporidium muris
Oocyst
Digital image processing
Morphological parameter
