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深低温停循环脑保护大鼠模型的建立 被引量：7

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摘要 目的建立一种新的深低温停循环(DHCA)脑缺血/保护大鼠模型。方法SD大鼠40只,分为实验组和对照组。麻醉后气管插管接小动物呼吸机,股动脉置管测动脉压力,监测心电、体温、血氧饱和度和血气分析,用冰块将大鼠体温降至21℃。实验组阻断双侧颈总动脉60min(对照组不阻断颈总动脉)后逐渐将体温恢复正常。结果实验组4只大鼠复苏失败死亡,16只大鼠成活,死亡率为20%,4h后Longa5级评分标准评定动物神经系统损伤为1～2级,24h后无神经系统受损表现;对照组无大鼠死亡,4h后Longa评分为0级。结论本实验方法制作的大鼠模型可以用于深低温停循环脑缺血/保护的研究。
作者 陶运明 莫绪明 谷兴琳 彭卫 戚继荣 顾海涛 张儒舫 钱龙宝
机构地区 南京医科大学附属南京儿童医院心胸外科
出处 《江苏医药》 CAS CSCD 北大核心 2005年第7期536-538,共3页 Jiangsu Medical Journal
关键词 深低温停循环 大鼠模型 脑保护 神经系统损伤 神经系统受损 颈总动脉 股动脉置管 血氧饱和度 对照组 实验组 SD大鼠 气管插管 动脉压力 血气分析 体温恢复 评分标准 实验方法 脑缺血 呼吸机 小动物 麻醉后 不阻断 死亡率
分类号 R654.1 [医药卫生—外科学] R285.5 [医药卫生—中药学]
  • 相关文献

参考文献6

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二级参考文献8

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共引文献17

同被引文献55

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引证文献7

二级引证文献23

江苏医药
2005年 第7期

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