摘要
The molecular variation of satellite DNA associated with Tobacco curly shoot virus (TbCSV) was compared by DNA sequencing of progenies of TbCSV DNA in Nicotiana benthamiana and N. glutinosa plants agroinoculated with infectious clones of TbCSV and its associated DNA. Comparisons indicated that TbCSV DNA populations had the higher levels of mutation frequency in N. glutinosa than that in N. benthamiana, and mutations generated in N. benthamiana were found upstream or downstream of C1 ORF whereas those in N. glutinosa were mostly located in A-rich region. No mutation was found in C1 ORF and satellite conserved region of DNA. The biological significance of the mutation accumulation and its role in disease epidemic were discussed.
The molecular variation of satellite DNA associated with Tobacco curly shoot virus (TbCSV) was compared by DNA sequencing of progenies of TbCSV DNA in Nicotiana benthamiana and N. glutinosa plants agroinoculated with infectious clones of TbCSV and its associated DNA. Comparisons indicated that TbCSV DNA populations had the higher levels of mutation frequency in N. glutinosa than that in N. benthamiana, and mutations generated in N. benthamiana were found upstream or downstream of C1 ORF whereas those in N. glutinosa were mostly located in A-rich region. No mutation was found in C1 ORF and satellite conserved region of DNA. The biological significance of the mutation accumulation and its role in disease epidemic were discussed.
出处
《病毒学报》
CAS
CSCD
北大核心
2005年第4期308-310,共3页
Chinese Journal of Virology
基金
国家杰出青年基金(No30125032)
国家自然科学基金(No30370927)资助项目
关键词
烟草曲茎病毒
卫星DNA
变异
本氏烟
心叶烟
<Keyword>Tobacco curly shoot virus
satellite DNA
variation
Nicotiana benthamiana
N. glutinosa