摘要
目的探讨p16基因及增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)在喉鳞状细胞癌中的表达及其临床意义。方法应用免疫组织化学技术SP法检测抑癌基因p16蛋白及PCNA在40例喉鳞状细胞癌中的表达,并与癌旁组织和正常喉组织对比,结合喉鳞状细胞癌的临床病理特征进行分析。结果在喉鳞状细胞癌中p16阳性率(50.0%)明显低于癌旁组织(76.0%)和正常组织(100.0%,P<0.05);Ⅰ、Ⅱ期喉癌中p16阳性率明显高于Ⅲ、Ⅳ期。喉鳞状细胞癌中PCNA阳性率为55.0%,明显高于癌旁组织(32.0%)和正常组织(10.0%,P<0.05);Ⅰ、Ⅱ期喉鳞状细胞癌中PCNA表达明显低于Ⅲ、Ⅳ期(P<0.05)。p16阳性与阴性患者生存期≥5年的比率分别为66.7%和33.3%,P<0.05;PCNA阳性与阴性患者生存期≥5年的比率分别为37.5%和62.5%,P<0.05。结论p16和PCNA分别是喉鳞状细胞癌进展及预后判断的独立指标;p16和PCNA联合检测对喉鳞状细胞癌的预后评估可能更有价值。
OBJECTIVE To study of the expres- sion and its clinical significance of p16 and proliferatingcell nuclear antigen(PCNA) in laryngeal squamous cellcarcinoma (LSCC). METHODS The expression of p16and PCNA were detected using immunohistochemistrymethod in tumor tissues of 40 patients with laryngealsquamous cell carcinoma. Their results were comparedto that in adjacent of cancer and normal tissues.RESULTS The positive expression rate of p16 in LSCCtissues was 50.0 % which was significantly lowerthan that in adjacent tissues (76.0 % ) and nor-mal tissues(100.0 %,P < 0.05).The positive ex-pression rate of p16 in LSCC tissues of patients withpTNM Ⅰ,Ⅱ stages was significantly higher than that ofpatients with pTNM Ⅲ,Ⅳ stages(P < 0.05).The posi-tive expression rate of PCNA in LSCC tissues was 55.0%which was significantly higher than that in adjacent tis-sues (32.0 %) and normal tissues(10.0 %)(P < 0.05).The positive expression rate of PCNA in LSCC tissuesof patients with pTNM Ⅰ,Ⅱ stages was lower than thatof patients with pTNM Ⅲ,Ⅳ stages(P < 0.05). The sur-vival times ≥ 5 years of patients with p16 positive andp16 negative was 66.7 % and 33.3 % respectively(P <0.05). The survival times ≥ 5 years of patients withPCNA positive and PCNA negative was 37.5 % and 62.5 %respectively(P < 0.05). CONCLUSION p16 and PCNA wereindependent markers of progression and prognosis of pa-tients with LSCC. The p16 and PCNA detected together maybe more helpful to predict the prognosis of patients with LSCC.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2005年第6期347-349,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
