摘要
目的研究不同剂量镉对大鼠钠钾ATP酶(Na+K+ATPase)和钙ATP酶(Ca+ATPase)的影响,以探讨镉中毒的细胞分子学机制。方法大鼠按体重随机分成4组,第1组为对照组,皮下注射0.9%氯化钠,第2～4组为镉染毒组,分别皮下注射3,5,7μmol/kg的氯化镉溶液,注射容量均为2ml/kg。染毒6周后,测定尿中乳酸脱氢酶(LDH)、尿蛋白、尿镉和肝、肾组织中Na+K+ATPase及Ca2+ATPase的活力。结果随着镉染毒剂量的增加,各组大鼠尿LDH、尿蛋白和尿镉含量均逐渐递增,呈现明显的剂量-效应关系;同时,肝、肾组织中的Na+K+ATPase和Ca2+ATPase活力也随之增加。结论随着镉染毒剂量的增加,大鼠肝、肾组织损伤逐渐加重,同时伴随着细胞膜上Na+K+ATPase和Ca2+ATPase的变化,这可能与细胞内钙稳态的破坏有关。
Objective To study the effects of the different doses of CdCl_2 on Na^+-K^+-ATPase and Ca^(2+)-ATPase in liver and kidney of rats in order to study the cell molecular mechanism of cadmium-induced damage. Methods The Wistar rats were divided into four groups by weight at random.The first group was the control group which was given subcutaneous injection of 0.9% NaCl.The second to fourth groups were respectively given subcutaneous injection of 3,5,(7 μmol/kg) CdCl_2.The capacity of injection is all (2 ml/kg).6 weeks after administration,the urine samples were collected and the rats were anatomized to get the tissue samples of renal cortex and liver.The LDH,contents of cadmium and protein in the rat urine and the activities of Na^+-K^+-ATPase and Ca^(2+)-ATPase in liver and renal cortex were determined. Results The results showed that compared with the control group the activities of Na^+-K^+-ATPase and Ca^(2+)-ATPase in the liver,kidney and urinary LDH,protein and contents of cadmium of the rats administrated with CdCl_2 increased significantly with a dose-response relationship. Conclusions As the dose injected increases,the tissue is damaged gradually accompanied with the change of activities of Na^+-K^+-ATPase and Ca^(2+)-ATPase in cell membrane.It might be related with the destruction of intracellular calcium homeostasis.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2005年第4期197-199,共3页
Industrial Health and Occupational Diseases
基金
国家自然科学基金资助(编号30371200)
