摘要
目的:观察抗菌剂作用后变形链球菌生物膜细胞活力。方法:将已形成24h变形链球菌生物膜分别用1000μg/ml、500μg/ml、100μg/ml、50μg/ml、0μg/ml红霉素药液作用3h后取出,对这些生物膜分别进行两种不同的处理。A组将生物膜继续培养后,用死菌活菌荧光染色方法结合激光共聚焦扫描显微镜进行观察;B组将生物膜细胞刮下接种到培养基上,革兰染色观察。结果:A组每个标本均重新形成较完整的生物膜;B组每个标本均发现有变形链球菌生长。结论:变形链球菌生物膜被高浓度抗菌剂作用后,膜中存活的细菌仍能分裂繁殖,并可修复已损伤的生物膜。
Objective:To observe the vitability of cells in the S. mutans biofilm after being treated with antimicobials.Method:Cultivated 24 h S. mutans biofilms were treated 3 h by erythromycin solution with the concentration of 1000 μg/ml、500 μg/ml、100 μg/ml、50 μg/ml、0 μg/ml.Then these biofilms divided into two groups were treated immediately with different ways. A group biofilms were cultivated 24 h again, then these biofilms were visualized by fluorescent stainning with LIVE/DEAD bacterial vitability kit and were observed by confocal laser scanning microscopy. B group biofilms cells were scraped and inoculated for cultivation, then observed by gram stainning method.Result:A group biofilms could generate biofilm once again. The biofilms cells in B group still could grow.Conclusion:While S. mutans biofilms were treated by antimicobials with high concentration, survived S. mutans in the biofilm were still schizogenous and able to repair damaged biofilm.
出处
《临床口腔医学杂志》
2005年第7期387-389,共3页
Journal of Clinical Stomatology
基金
教育部留学回国人员科研启动基金资助项目(01GJHG02)
上海市科委基础性研究计划(04JC14066)
关键词
变形链球菌
生物膜
抗菌剂
细胞活力
streptococcus mutans
biofilm
antimicrobials
cell vitability
