摘要
目的用3种不同途径感染Fukaya株弓形虫速殖子,观察弓形虫感染小鼠慢性期病变特点及虫体在脑内的成囊过程,以期寻求一个稳定、易建的慢性感染动物模型,为弓形虫病的病理诊断提供依据,并对弓形虫的致病机理加深理解。方法弓形虫Fukaya株速殖子(5×104个)分别以腹腔、皮下和口服途径感染小鼠,给适量药物使之形成慢性感染,并与不给药组做对照,于感染后第3d、6d1、4d2、1d、28d、42d和90d,取脑进行间接免疫酶染色,统计脑内虫体数量及形成包囊情况。结果不给药组第3d、6d与给药组第6d,3种感染途径的腹腔含虫数比较:均为腹腔感染>皮下感染>经口感染。比较小鼠脑内虫体分布:腹腔感染组,第28d的脑内虫体达到高峰,此时脑内包囊最多。皮下感染组,第21d的脑内虫体最多,脑内包囊也多。经口感染组,第21d脑内虫体最多,但脑内偶见包囊。经口感染Fukaya株速殖子似不易成囊。经腹腔、皮下和口服感染虫体的小鼠存活率在第42d分别为100%、66%、80%。结论弓形虫感染慢性期小鼠脑多被累及。腹腔与皮下感染弓形虫Fukaya株速殖子比口服易成囊,经腹腔感染方式建立弓形虫慢性感染动物模型较稳定。
By using three different routes of infection with Fukaya strain of Toxoplasma gondii, the characteristics of chronic lesions and the formation of cysts in the brain of the infected mice were observed in order to set up a chronic animal model for the studies on the pathogenesis and diagnosis of toxoplasmosis. Mice were infected with tachyzoites of Fukaya strain (5 x10^4) by intraperitoneal inoculation (IP), subcutaneous injection (SC) and per-oral gavage (PO), and the chronic infection was established after treatment with certain amount of drugs with the control group of mice without giving any drugs. The brain samples of the infected mice were taken at 3, 6, 14, 21, 28, 42 and 90 days after infection and the parasites were detected by indirect immunoenzymatic staining. The count of parasites in the brain was determined and the cyst formation in each case was observed. It was demonstrated that the count of parasites in the peritoneal cavity of the IP-infected mice was found to be the highest one among these 3 groups of mice at 3 and 6 days after infection,and showing a tendency of IP>SC>PO.The count of parasites in the brain of IP-infected mice reached its peak value at 28 th day after infection, so did as the cyst count in the brain, and those of the SC-infected mice showed its peak in the brain at 21?th day after infection, also for the cyst count in the brain. In PO-infected mice, parasite count peaked also at 21?th day after infection, but cysts in the brain were seldom found, suggesting that cysts could not be easily formed by route of per-oral lavage. The (iviabilities) of mice at 42 days after infection by these 3 routes were 100%, 66% and 80% respectively. It is concluded that brains of mice are usually infected during chronic stage of the toxoplasma infection, in which the cysts are easily formed in case of infection infected by peritoneal and subcutaneous routes than those infected by per-oral route.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2005年第7期616-619,共4页
Chinese Journal of Zoonoses
基金
天津自然科学基金资助(NO:973609311)
关键词
弓形虫
感染途径
包囊
<Keyword>Toxoplasma gondii
infected way
cyst
