摘要
目的探讨急、慢性病毒性心肌炎和感染性扩张型心肌病小鼠心肌细胞凋亡的动态变化。方法以柯萨奇病毒B3感染Balb/c小鼠建立急、慢性心肌炎和扩张型心肌病的系列动物模型,同期设正常对照;经组织病理学方法和超声心动图验证模型后,以缺口末端标记法(TUNEL)检测各组小鼠心肌细胞凋亡。结果各期感染小鼠心肌细胞凋亡率较同期对照组明显增高,心肌细胞凋亡率依次分别为(8.2±1.4)%vs(1.3±0.5)%,(15.3±1.1)%vs(2.7±0.7)%,(13.9±2.1)%vs(3.1±0.6)%,(均P<0.01)。结论心肌细胞凋亡贯穿病毒性心脏病全程,可能对其病程和预后产生深远影响。
Purpose To investigate dynamic alteration of apoptosis in myocardium of mice with acute,chronic myocarditis and dilated cardiomyopathy. Methods Balb/c mice infected with coxsackievirus B3 were used to establish animal models of acute,chronic myocarditis and dilated cardiomyopathy,while uninfected animals were also prepared and served as controls.After verification of models by histopathological methods and echocardiography,cardiomyocyte apoptosis in each group of mice was detected by terminal deoxynucleotidyltransferase -mediated dUTP-biotin nick end-labeling (TUNEL) assay. Results Cardiomyocyte apoptosis in all infected group of mice were significantly increased in comparison with corresponding controls.The apoptotic rate was [(8.2±1.4)% vs (1.3±0.5)%,P<0.01],[(15.3±1.1)% vs (2.7±0.7)%,P<0.01] and [(13.9±2.1)% vs (3.1±0.6)%,P<0.01],respectively. Conclusions Cardiomyocyte apoptosis involves in the whole pathological course,and may play a role in the pathogenesis and prognosis of viral heart diseases.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2005年第4期388-390,F002,共4页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金资助项目(30271665)
